连接到 ω-官能化 PEG 刷的脂质体，并诱导 PEG 刷支撑的平面脂质双层的形成。

Liposomes tethered to omega-functional PEG brushes and induced formation of PEG brush supported planar lipid bilayers.

机构信息

Swiss Federal Institute of Technology, Laboratory for Surface Science and Technology, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland.

出版信息

Langmuir. 2009 Dec 1;25(23):13534-9. doi: 10.1021/la902039g.

DOI:10.1021/la902039g

PMID:19736981

Abstract

Self-assembly of planar supported lipid bilayers on top of hydrophilic polymer brushes is a desirable alternative to solid supported lipid bilayers and covalently tethered lipid bilayers for applications like sensing on transmembrane proteins which require a large aqueous volume between membrane and substrate. We present a simple dip-and-rinse method to produce poly(ethylene glycol) (PEG) brushes with sparse positively charged hydrophobic tethers, using poly(l-lysine)-graft-poly(ethylene glycol)-quaternary ammonium compound copolymers. The interaction of such polymer coatings with liposomes of different compositions and the conditions for formation of planar lipid bilayers of extraordinarily high fluidity on top of the >10 nm thick reservoir by liposome self-assembly and sequentially triggered rupture are investigated.

摘要

在亲水聚合物刷上自组装平面支撑脂质双层是一种替代固相支撑脂质双层和共价键合脂质双层的理想方法，适用于需要在膜和基底之间有大的水体积的跨膜蛋白传感等应用。我们提出了一种简单的浸涂-冲洗方法，使用聚（赖氨酸）-接枝-聚（乙二醇）-季铵盐共聚物制备具有稀疏正电荷疏水性连接体的聚乙二醇（PEG）刷。研究了这种聚合物涂层与不同组成的脂质体的相互作用，以及通过脂质体自组装和顺序触发破裂在 >10nm 厚的储层上形成具有极高流动性的平面脂质双层的条件。