Department of Bio-Sciences, Jamia Millia Islamia, New Delhi 110 025, India.
J Photochem Photobiol B. 2009 Nov 9;97(2):87-93. doi: 10.1016/j.jphotobiol.2009.08.006. Epub 2009 Aug 18.
Recent research has shown that, like porphyrins, phycocyanin (PC) too can produce singlet oxygen upon excitation with the appropriate radiation and hence could be useful in photodynamic therapy (PDT) for cancer. Unlike porphyrins, PC has the advantage of being a non-toxic, non-carcinogenic, soluble protein. However, the challenge would be to target the fluorescent phycobiliprotein to malignant cells. We report here that the tumor-specific lectin, jacalin, binds PC specifically in a carbohydrate-independent manner and with affinities better than that for porphyrins. Hence the lectin could prove to be a useful carrier for targeted delivery of PC. The interaction involves both ionic and hydrophobic interactions and more than one contact site.
最近的研究表明，藻蓝蛋白（PC）与卟啉类似，在适当的辐射激发下也能产生单线态氧，因此可用于癌症的光动力疗法（PDT）。与卟啉不同的是，PC 具有无毒、非致癌、可溶性蛋白的优点。然而，挑战在于将荧光藻胆蛋白靶向恶性细胞。我们在这里报告，肿瘤特异性凝集素，荆豆凝集素，以非碳水化合物依赖的方式特异性结合 PC，亲和力优于卟啉。因此，该凝集素可能成为 PC 靶向递送的有用载体。这种相互作用涉及离子和疏水相互作用以及不止一个接触点。
