Colorado State University, Fort Collins 80523, CO, USA.
Anim Genet. 2009 Dec;40(6):986-8. doi: 10.1111/j.1365-2052.2009.01946.x. Epub 2009 Sep 10.
Two half-sib families of backcross progeny were produced by mating F(1) Line 1 Hereford (L1) x composite gene combination (CGC) bulls with L1 and CGC cows. Feed intake and periodic weights were measured for 218 backcross progeny. These progenies were genotyped using 232 microsatellite markers that spanned the 29 BTA. Progeny from L1 and CGC females was analysed separately using composite interval mapping to find quantitative trait loci (QTL) affecting daily dry matter intake (DMI), average daily gain (ADG), feed conversion (FCR) and residual feed intake (RFI). Results from both backcrosses were pooled to find additional QTL. In the backcross to L1, QTL were detected for RFI and DMI on BTA11, FCR on BTA16, and ADG on BTA9. In the backcross to CGC, QTL were detected for RFI on BTA10, FCR on BTA12 and 16 and ADG on BTA15 and 17. After pooling, QTL were detected for RFI on BTA 2, 6, 7, 10, 11, 13 and 16; for FCR on BTA 9, 12, 16, 17 and 21; for ADG on BTA 9, 14, 15, 17; and for DMI on BTA 2, 5, 6, 9, 10, 11, 20 and 23.
两个半同胞家系的回交后代是通过交配 F(1) 代 1 号荷斯坦(L1)x 复合基因组合(CGC)公牛与 L1 和 CGC 母牛产生的。对 218 头回交后代进行了饲料摄入量和定期体重测量。这些后代使用 232 个微卫星标记进行了基因分型,这些标记跨越了 29 个 BTA。使用复合区间作图法分别分析来自 L1 和 CGC 雌性的后代,以找到影响每日干物质摄入量(DMI)、平均日增重(ADG)、饲料转化率(FCR)和剩余饲料摄入量(RFI)的数量性状基因座(QTL)。将两个回交的结果合并,以找到其他 QTL。在回交至 L1 的后代中,在 BTA11 上检测到 RFI 和 DMI 的 QTL,在 BTA16 上检测到 FCR 的 QTL,在 BTA9 上检测到 ADG 的 QTL。在回交至 CGC 的后代中,在 BTA10 上检测到 RFI 的 QTL,在 BTA12 和 16 上检测到 FCR 的 QTL,在 BTA15 和 17 上检测到 ADG 的 QTL。合并后,在 BTA 2、6、7、10、11、13 和 16 上检测到 RFI 的 QTL;在 BTA 9、12、16、17 和 21 上检测到 FCR 的 QTL;在 BTA 9、14、15、17 上检测到 ADG 的 QTL;在 BTA 2、5、6、9、10、11、20 和 23 上检测到 DMI 的 QTL。