高效液相色谱-紫外检测法与液相色谱-串联质谱法测定慢性髓系白血病患者伊马替尼血药浓度：方法学比较。
Determination of imatinib plasma levels in patients with chronic myeloid leukemia by high performance liquid chromatography-ultraviolet detection and liquid chromatography-tandem mass spectrometry: methods' comparison.
机构信息
Department of Medicine, Jordan University, Amman, Jordan.
出版信息
Leuk Res. 2010 Jun;34(6):714-7. doi: 10.1016/j.leukres.2009.08.005. Epub 2009 Sep 9.
The aims of this study were to validate and compare HPLC and LCMSMS analytical methods and their applicability for the quantitation of imatinib in human plasma. A total of 50 patients with chronic myeloid leukemia (CML) in chronic phase (CP) receiving 400 mg/day imatinib were enrolled in the study. Drug levels were determined by HPLC-UV and LCMSMS. HPLC intra-day accuracy ranged from 100.51 to 103.19%. LCMSMS accuracy ranged from 89.72 to 106.29%. The correlation coefficient between both methods was r(2)=0.96. HPLC can be used for imatinib levels' determinations in patients accurately and precisely.
本研究的目的是验证和比较 HPLC 和 LCMSMS 分析方法及其在人血浆中定量测定伊马替尼的适用性。共有 50 名接受每天 400 毫克伊马替尼治疗的慢性髓性白血病（CML）慢性期（CP）患者入组本研究。通过 HPLC-UV 和 LCMSMS 测定药物水平。HPLC 日内准确度范围为 100.51%至 103.19%。LCMSMS 准确度范围为 89.72%至 106.29%。两种方法之间的相关系数为 r(2)=0.96。HPLC 可用于准确、精密地测定患者的伊马替尼水平。
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