Pawelec G, Schaudt K, Rehbein A, Olive D, Bühring H J
Second Department of Internal Medicine, University Medical Clinic, Tübingen, Federal Republic of Germany.
Cell Immunol. 1990 Sep;129(2):385-93. doi: 10.1016/0008-8749(90)90214-c.
Requirements for stimulating autocrine proliferation of human T cell clones expressing either alpha/beta or gamma/delta antigen receptors via the "alternative" CD2 pathway have been examined using a large set of monoclonal antibodies (mAb). In the presence of autologous accessory cells (AC, B-lymphoblastoid cell lines) 2 of 13 single CD2 mAb (CLB-T11.1/1 and 6F10.3) stimulated proliferation of gamma/delta but not alpha/beta cells. Interleukin (IL) 1 or IL 6 did not substitute for AC in stimulating gamma/delta clones. Addition of CD28 mAb YTH 913.12 with the CD2 mAb did not result in stimulation of any alpha/beta clones. In the absence of AC, none of the CD2 mAb singly could stimulate any T cell clones, but pairs of mAb directed to different epitopes of CD2 (CLB-T11.1/1 + CLB- T11.2/1 or 6F10.3 + 39C1.5) stimulated both alpha/beta and gamma/delta clones. In both cases, stimulation was reduced by the presence of CD3 mAb. These results confirm that the established AC-independent alternative pathway of T cell activation, which requires binding of two separate epitopes of CD2, operates in both gamma/delta and alpha/beta T cells, and further suggest that an additional pathway initiated by binding of a single CD2 epitope in the presence of AC is exclusively operational in gamma/delta cells.
通过“替代”CD2途径刺激表达α/β或γ/δ抗原受体的人T细胞克隆自分泌增殖的要求，已使用大量单克隆抗体（mAb）进行了研究。在自体辅助细胞（AC，B淋巴细胞系）存在的情况下，13种单一CD2 mAb中的2种（CLB-T11.1/1和6F10.3）刺激了γ/δ细胞的增殖，但未刺激α/β细胞。白细胞介素（IL）1或IL 6在刺激γ/δ克隆时不能替代AC。将CD28 mAb YTH 913.12与CD2 mAb一起添加不会刺激任何α/β克隆。在没有AC的情况下，没有一种CD2 mAb能单独刺激任何T细胞克隆，但针对CD2不同表位的mAb对（CLB-T11.1/1 + CLB-T11.2/1或6F10.3 + 39C1.5）刺激了α/β和γ/δ克隆。在这两种情况下，CD3 mAb的存在都会降低刺激作用。这些结果证实，已确立的不依赖AC的T细胞激活替代途径，需要CD2的两个单独表位结合，在γ/δ和α/β T细胞中均起作用，并且进一步表明，在AC存在下由单个CD2表位结合引发的另一条途径仅在γ/δ细胞中起作用。
