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通过限制性片段长度多态性(RFLP)分析对挪威奶山羊的山羊β-珠蛋白基因簇进行特征鉴定。

Characterization by RFLP analysis, of the Caprine beta-globin gene cluster in Norwegian dairy goats.

作者信息

Bergersen O, Braend M

机构信息

Norwegian College of Veterinary Medicine, Department of Animal Genetics, Oslo.

出版信息

Hemoglobin. 1990;14(1):87-102. doi: 10.3109/03630269009002257.

Abstract

Restriction fragment length polymorphism analysis has been used to characterize the beta-globin cluster in Norwegian dairy goats. Several polymorphic Taq I restriction sites were identified by double digestions with Bam HI, Hind III, and Eco RI, and restriction maps of the different beta C, beta A and beta F alleles were established. Allelic association between the previously established beta-globin types at the protein level and specific Taq I alleles was found. Furthermore, a preferential association between certain RFLP haplotypes and beta-globin types was observed.

摘要

限制性片段长度多态性分析已被用于鉴定挪威奶山羊的β-珠蛋白基因簇。通过用Bam HI、Hind III和Eco RI进行双酶切,鉴定出了几个多态性Taq I限制性位点,并建立了不同βC、βA和βF等位基因的限制性图谱。发现了先前在蛋白质水平上确定的β-珠蛋白类型与特定Taq I等位基因之间的等位基因关联。此外,还观察到某些RFLP单倍型与β-珠蛋白类型之间存在优先关联。

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