Rebl Alexander, Anders Eckhard, Wimmers Klaus, Goldammer Tom
Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere (FBN), Molecular Biology Research Unit, Dummerstorf, Germany.
Genes Genet Syst. 2009 Jun;84(3):239-43. doi: 10.1266/ggs.84.239.
In eukaryotic cells, intracellular transport is mediated by coated vesicular carriers. Coat proteins I (COPI) vesicles are involved in the retrograde transport from Golgi apparatus to the endoplasmic reticulum. The COPI complex is composed of ADP-ribosylation factor 1 and coatomer comprising seven subunits, termed alpha-zeta. We isolated and characterised a cDNA sequence from rainbow trout homolog to delta-subunit of COPI complex (delta-COP). Trout delta-COP gene encodes a protein of 509 aa including a characteristic Mu homology domain. Searches at the Ensemble Genome browser identified three additional teleostean delta-COP-like sequences from pufferfish, rice fish, and stickleback. Sequence identity of piscine delta-COP protein sequences is greater than 84%. Moreover, a phylogenetic analysis indicates that delta-COP protein sequences are strongly conserved among vertebrate species. delta-COP homologue is ubiquitously expressed in trout tissues. Quantitative Real-Time RT-PCR revealed that delta-COP is differentially expressed in liver and gill tissue of two rainbow trout strains, the freshwater strain STEELHEAD and the brackish water-adapted strain BORN.
在真核细胞中，细胞内运输由包被囊泡载体介导。包被蛋白I（COPI）囊泡参与从高尔基体到内质网的逆行运输。COPI复合体由ADP核糖基化因子1和包含七个亚基（称为α-ζ）的包被蛋白复合体组成。我们从虹鳟鱼中分离并鉴定了与COPI复合体δ亚基（δ-COP）同源的cDNA序列。鳟鱼δ-COP基因编码一个509个氨基酸的蛋白质，包括一个特征性的Mu同源结构域。在Ensemble基因组浏览器中搜索发现，来自河豚、食蚊鱼和棘鱼的另外三个硬骨鱼δ-COP样序列。鱼类δ-COP蛋白序列的同一性大于84%。此外，系统发育分析表明，δ-COP蛋白序列在脊椎动物物种中高度保守。δ-COP同源物在鳟鱼组织中普遍表达。实时定量RT-PCR显示，δ-COP在两个虹鳟鱼品系（淡水品系STEELHEAD和适应咸淡水的品系BORN）的肝脏和鳃组织中差异表达。
