Suppr超能文献

大鼠脑星形胶质细胞中的瘦素受体 mRNA。

Leptin receptor mRNA in rat brain astrocytes.

机构信息

Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA.

出版信息

Peptides. 2009 Dec;30(12):2275-80. doi: 10.1016/j.peptides.2009.08.023. Epub 2009 Sep 9.

Abstract

We recently reported that mouse astrocytes express leptin receptors (ObR), and that obesity induces upregulation of astrocytic ObR. To provide further evidence of the importance of astrocytic ObR expression, we performed double-labeling fluorescent in situ hybridization (FISH) and immunohistochemistry in the rat hypothalamus. Laser confocal microscopic image analysis showed that ObR mRNA was present in glial fibrillary acidic protein (+) cells that show distinctive astrocytic morphology as well as in neurons. In addition to the presence of ObR mRNA, ObR protein was shown in both astrocytes and neurons in the rat hypothalamus by double-labeling immunohistochemistry. In cultured rat C6 astrocytoma cells treated with different doses of lipopolysaccharide for 6h, the mRNA for ObRa or ObRb did not show significant changes, as measured by quantitative RT-PCR. However, the protein expression of both ObRa and ObRb, determined by Western blotting, was increased after the C6 cells were treated with either lipopolysaccharide or tumor necrosis factor-alpha. The results indicate that astrocytic ObR expression is present in rats as well as mice, and that it probably plays a role in the neuroinflammatory response.

摘要

我们最近报道了小鼠星形胶质细胞表达瘦素受体(ObR),肥胖诱导星形胶质细胞 ObR 的上调。为了提供星形胶质细胞 ObR 表达重要性的进一步证据,我们在大鼠下丘脑进行了双标荧光原位杂交(FISH)和免疫组织化学研究。激光共聚焦显微镜图像分析显示,ObR mRNA 存在于胶质纤维酸性蛋白(+)细胞中,这些细胞具有独特的星形胶质细胞形态,也存在于神经元中。通过双标免疫组织化学,除了存在 ObR mRNA 外,还在大鼠下丘脑的星形胶质细胞和神经元中显示出 ObR 蛋白。在培养的大鼠 C6 星形细胞瘤细胞中,用不同剂量的脂多糖处理 6 小时后,通过定量 RT-PCR 测量,ObRa 或 ObRb 的 mRNA 没有显示出明显变化。然而,用脂多糖或肿瘤坏死因子-α处理 C6 细胞后,通过 Western blot 测定,ObRa 和 ObRb 的蛋白表达均增加。结果表明,星形胶质细胞 ObR 表达不仅存在于小鼠中,也存在于大鼠中,并且可能在神经炎症反应中发挥作用。

相似文献

1
Leptin receptor mRNA in rat brain astrocytes.
Peptides. 2009 Dec;30(12):2275-80. doi: 10.1016/j.peptides.2009.08.023. Epub 2009 Sep 9.
2
Role of astrocytic leptin receptor subtypes on leptin permeation across hCMEC/D3 human brain endothelial cells.
J Neurochem. 2010 Dec;115(5):1288-98. doi: 10.1111/j.1471-4159.2010.07028.x. Epub 2010 Oct 26.
3
Obesity induces functional astrocytic leptin receptors in hypothalamus.
Brain. 2009 Apr;132(Pt 4):889-902. doi: 10.1093/brain/awp029. Epub 2009 Mar 17.
4
Astrocyte leptin receptor (ObR) and leptin transport in adult-onset obese mice.
Endocrinology. 2008 Jun;149(6):2798-806. doi: 10.1210/en.2007-1673. Epub 2008 Feb 21.
5
Upregulation of astrocytic leptin receptor in mice with experimental autoimmune encephalomyelitis.
J Mol Neurosci. 2013 Mar;49(3):446-56. doi: 10.1007/s12031-012-9825-2. Epub 2012 Jun 10.
9
Leptin receptors are developmentally regulated in rat pituitary and hypothalamus.
Mol Cell Endocrinol. 2003 Nov 28;210(1-2):1-8. doi: 10.1016/j.mce.2003.09.003.
10
Protective role of astrocytic leptin signaling against excitotoxicity.
J Mol Neurosci. 2013 Mar;49(3):523-30. doi: 10.1007/s12031-012-9924-0. Epub 2012 Nov 23.

引用本文的文献

1
Impact of neuroinflammation on brain glutamate and dopamine signalling in schizophrenia: an update.
Metab Brain Dis. 2025 Feb 5;40(2):119. doi: 10.1007/s11011-025-01548-3.
2
Discovery of a Novel Dual Targeting Peptide for Human Glioma: From Simulation to Acting as Targeting Ligand.
Adv Pharm Bull. 2024 Jul;14(2):453-468. doi: 10.34172/apb.2024.033. Epub 2024 Mar 10.
3
Lateral parabrachial nucleus astrocytes control food intake.
Front Endocrinol (Lausanne). 2024 Jun 3;15:1389589. doi: 10.3389/fendo.2024.1389589. eCollection 2024.
5
From Synapses to Circuits, Astrocytes Regulate Behavior.
Front Neural Circuits. 2022 Jan 4;15:786293. doi: 10.3389/fncir.2021.786293. eCollection 2021.
6
Glial Modulation of Energy Balance: The Dorsal Vagal Complex Is No Exception.
Int J Mol Sci. 2022 Jan 16;23(2):960. doi: 10.3390/ijms23020960.
7
Hypothalamic Microglial Heterogeneity and Signature under High Fat Diet-Induced Inflammation.
Int J Mol Sci. 2021 Feb 24;22(5):2256. doi: 10.3390/ijms22052256.
8
Intracellular interplay between cholecystokinin and leptin signalling for satiety control in rats.
Sci Rep. 2020 Jul 20;10(1):12000. doi: 10.1038/s41598-020-69035-6.
10
Deletion of astrocytic BMAL1 results in metabolic imbalance and shorter lifespan in mice.
Glia. 2020 Jun;68(6):1131-1147. doi: 10.1002/glia.23764. Epub 2019 Dec 13.

本文引用的文献

1
Cerebral microvascular IL15 is a novel mediator of TNF action.
J Neurochem. 2009 Nov;111(3):819-27. doi: 10.1111/j.1471-4159.2009.06371.x. Epub 2009 Aug 31.
2
Obesity induces functional astrocytic leptin receptors in hypothalamus.
Brain. 2009 Apr;132(Pt 4):889-902. doi: 10.1093/brain/awp029. Epub 2009 Mar 17.
4
Astrocyte leptin receptor (ObR) and leptin transport in adult-onset obese mice.
Endocrinology. 2008 Jun;149(6):2798-806. doi: 10.1210/en.2007-1673. Epub 2008 Feb 21.
5
Developmental changes of leptin receptors in cerebral microvessels: unexpected relation to leptin transport.
Endocrinology. 2008 Mar;149(3):877-85. doi: 10.1210/en.2007-0893. Epub 2007 Nov 26.
6
Opposing effects of proteasomes and lysosomes on LIFR: modulation by TNF.
J Mol Neurosci. 2007;32(1):80-9. doi: 10.1007/s12031-007-0017-4.
7
Adipokines and the blood-brain barrier.
Peptides. 2007 Jun;28(6):1317-30. doi: 10.1016/j.peptides.2007.04.023. Epub 2007 May 6.
8
Receptors for leptin and estrogen in the subcommissural organ of rabbits are differentially modulated by fasting.
Brain Res. 2006 Dec 8;1124(1):62-9. doi: 10.1016/j.brainres.2006.09.091. Epub 2006 Nov 7.
9
Gamma glutamyl transpeptidase is a dynamic indicator of endothelial response to stroke.
Exp Neurol. 2007 Jan;203(1):116-22. doi: 10.1016/j.expneurol.2006.07.023. Epub 2006 Sep 14.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验