Neurochirurgia Dipartimento di Scienze Chirurgiche Università di Pavia Fondazione IRCCS Policlinico S. Matteo, Pavia, Italy.
Cancer Lett. 2010 Apr 1;290(1):36-42. doi: 10.1016/j.canlet.2009.08.023. Epub 2009 Sep 12.
By qPCR we found that EDG3 and SHC3 were amplified in 60% of ependymomas but none in choroid plexus papillomas. In ependymomas EDG3 and SHC3 amplification increased Shc3 protein levels while EDG3 was less affected. Both proteins were co-immunoprecipitated from ependymoma and Shc3 was tyrosine phosphorylated thus presumably active. We showed by digestion with N-glycosidase-F that EDG3 was glycosylated indicating that EDG3 protein was not retained in the endoplasmic reticulum. The co-immunoprecipitation of Shc3 and EDG3 proteins from ependymomas with amplification of SHC3 and EDG3 genes suggests that the two proteins co-operate and are important for ependymomas in vivo.
通过 qPCR 我们发现，EDG3 和 SHC3 在 60%的室管膜瘤中被扩增，但在脉络丛乳头状瘤中均未被扩增。在室管膜瘤中，EDG3 和 SHC3 的扩增增加了 Shc3 蛋白水平，而 EDG3 的影响较小。这两种蛋白质都从室管膜瘤中被共免疫沉淀，并且 Shc3 被酪氨酸磷酸化，因此推测是活性的。我们通过用 N-糖苷酶 F 消化表明 EDG3 被糖基化，表明 EDG3 蛋白未在内质网中保留。具有 SHC3 和 EDG3 基因扩增的室管膜瘤中 Shc3 和 EDG3 蛋白的共免疫沉淀表明，这两种蛋白质相互合作，对体内的室管膜瘤很重要。
