Patrick Joyce E, Kearns Daniel B
Indiana University, Department of Biology, 1001 East Third Street, Bloomington, IN 47405, USA.
J Bacteriol. 2009 Nov;191(22):7129-33. doi: 10.1128/JB.00905-09. Epub 2009 Sep 11.
We redemonstrate that SwrA is essential for swarming motility in Bacillus subtilis, and we reassert that laboratory strains of B. subtilis do not swarm. Additionally, we find that a number of other genes, previously reported to be required for swarming in laboratory strains, are dispensable for robust swarming motility in an undomesticated strain. We attribute discrepancies in the literature to a lack of reproducible standard experimental conditions, selection for spontaneous swarming suppressors, inadvertent genetic linkage to swarming mutations, and auxotrophy.
我们再次证明，SwrA对枯草芽孢杆菌的群体运动至关重要，并且我们重申枯草芽孢杆菌的实验室菌株不会群体运动。此外，我们发现，先前报道在实验室菌株群体运动中所需的许多其他基因，对于未驯化菌株中强劲的群体运动是可有可无的。我们将文献中的差异归因于缺乏可重复的标准实验条件、对自发群体运动抑制子的选择、与群体运动突变的无意遗传连锁以及营养缺陷型。
