Pathak A, Patnaik S, Gupta K C
Institute of Genomics and Integrative Biology, Delhi University Campus, Delhi 110007, India.
Nucleic Acids Symp Ser (Oxf). 2009(53):57-8. doi: 10.1093/nass/nrp029.
Introduction of therapeutic genes into the cells of an organism in a safe and efficient way has become a challenging task in non-viral mediated gene therapy. Here, branched polyethylenimine (bPEI, 25 kDa) was converted into nanoparticles through electrostatic interactions with anionic polysaccharides (e.g. alginic acid, Al and hyaluronic acid, HA). A small library of PEI-Al and PEI-HA nanoparticles was synthesized by varying the amounts of anionic polysaccharides and evaluated in terms of their size, surface charge, cytotoxicity, transfection efficiency, etc. Both the series of nanoparticles exhibited higher cell viability and transfection efficiency as compared to native PEI and the standard transfection reagents. In vivo targeting efficacy of PEI-HA(4.6%) nanoparticles was examined in tumor induced mice.
以安全有效的方式将治疗性基因导入生物体细胞已成为非病毒介导基因治疗中的一项具有挑战性的任务。在此，通过与阴离子多糖（如海藻酸、Al和透明质酸、HA）的静电相互作用，将支化聚乙烯亚胺（bPEI，25 kDa）转化为纳米颗粒。通过改变阴离子多糖的量合成了一个PEI-Al和PEI-HA纳米颗粒的小型文库，并根据其尺寸、表面电荷、细胞毒性、转染效率等进行了评估。与天然PEI和标准转染试剂相比，这两个系列的纳米颗粒均表现出更高的细胞活力和转染效率。在肿瘤诱导小鼠中检测了PEI-HA(4.6%)纳米颗粒的体内靶向效果。
