Weiner Allon, Zauberman Nathan, Minsky Abraham
Department of Organic Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.
Nat Rev Microbiol. 2009 Oct;7(10):748-55. doi: 10.1038/nrmicro2206.
Double-strand DNA breaks (DSBs) are the most detrimental lesion that can be sustained by the genetic complement, and their inaccurate mending can be just as damaging. According to the consensual view, precise DSB repair relies on homologous recombination. Here, we review studies on DNA repair, chromatin diffusion and chromosome confinement, which collectively imply that a genome-wide search for a homologous template, generally thought to be a pivotal stage in all homologous DSB repair pathways, is improbable. The implications of this assertion for the scope and constraints of DSB repair pathways and for the ability of diverse organisms to cope with DNA damage are discussed.
双链DNA断裂（DSBs）是遗传物质所能承受的最有害的损伤，其修复不准确同样具有破坏性。根据普遍观点，精确的DSB修复依赖于同源重组。在此，我们综述了关于DNA修复、染色质扩散和染色体限制的研究，这些研究共同表明，全基因组范围内寻找同源模板（通常被认为是所有同源DSB修复途径中的关键阶段）是不太可能的。本文讨论了这一观点对DSB修复途径的范围和限制以及不同生物体应对DNA损伤能力的影响。
