Wakasaki H, Ooshima A
Department of Pathology, Wakayama Medical College, Japan.
Lab Invest. 1990 Sep;63(3):377-84.
Lysyl oxidase initiates the cross-linking of collagen and elastin by catalyzing the formation of the lysine-derived aldehyde. We cloned three hybridoma cell lines which secrete monoclonal antibodies to human lysyl oxidase. The localization of lysyl oxidase was investigated in various tissues and in cultured cells using an immunofluorescent antibody method. Antibodies showed a strong immunostaining in the aorta and dermal connective tissue suggesting a close relation to elastin and collagen. Fibroblasts, chondrocytes, and smooth muscle cells also yielded a marked positive immunoreaction as did a variety of nonfibroblastic cells such as endothelial cells, basal cells, biliary epithelial cells, and glomerular epithelial cells. In cultured cells, including human fibroblasts, an intense immunoreaction manifested as fine, filamentous structures in the cytoplasm. It is suggested that lysyl oxidase is associated with cytoskeletal protein.
赖氨酰氧化酶通过催化赖氨酸衍生醛的形成来启动胶原蛋白和弹性蛋白的交联。我们克隆了三个分泌抗人赖氨酰氧化酶单克隆抗体的杂交瘤细胞系。使用免疫荧光抗体法在各种组织和培养细胞中研究了赖氨酰氧化酶的定位。抗体在主动脉和真皮结缔组织中显示出强烈的免疫染色，表明与弹性蛋白和胶原蛋白密切相关。成纤维细胞、软骨细胞和平滑肌细胞以及各种非成纤维细胞如内皮细胞、基底细胞、胆管上皮细胞和肾小球上皮细胞也产生了明显的阳性免疫反应。在包括人成纤维细胞在内的培养细胞中，强烈的免疫反应表现为细胞质中的精细丝状结构。提示赖氨酰氧化酶与细胞骨架蛋白有关。
