甾体激素改变阴道毛滴虫完整滋养体中的 AMP 水解。
Steroid hormones alter AMP hydrolysis in intact trophozoites of Trichomonas vaginalis.
机构信息
Programa de Pós-Graduação em Biologia Celular e Molecular, Laboratório de Neuroquímica e Psicofarmacologia, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Avenida Ipiranga, 90619-900, Porto Alegre, RS, Brazil.
出版信息
Parasitol Res. 2009 Nov;105(6):1701-6. doi: 10.1007/s00436-009-1618-3. Epub 2009 Sep 16.
Trichomonas vaginalis infection may be influenced by the vaginal concentrations of estrogens. We have investigated the effects of 17beta-estradiol and dehydroepiandrosterone sulfate (DHEAS) on the ecto-5'-nucleotidase activity in fresh clinical (VP60) and in long-term-grown (30236 ATCC) isolates of T. vaginalis. In vitro exposure to DHEAS and 17beta-estradiol did not induce any changes in adenosine monophosphate (AMP) hydrolysis in these isolates. The treatment of parasites in the presence of DHEAS (0.01-1.0 microM) for 2 h inhibited AMP hydrolysis in VP60 isolate, whereas there were no significant changes in nucleotide hydrolysis in the presence of 17beta-estradiol. DHEAS and 17beta-estradiol (0.01-1.0 microM) for 2 h inhibited AMP hydrolysis in 30236 isolate. The 12 treatment with 0.1 microM DHEAS inhibited AMP hydrolysis, whereas 17beta-estradiol did not alter the nucleotide hydrolysis in VP60 isolate. Our findings have shown that the complex effect of steroid hormones and their receptors on T. vaginalis may promote changes in ecto-5'-nucleotidase activity during exposure to these hormones.
阴道滴虫感染可能受雌激素的阴道浓度影响。我们已经研究了雌二醇和硫酸脱氢表雄酮（DHEAS）对新鲜临床（VP60）和长期培养（30236 ATCC）阴道滴虫分离株的ecto-5'-核苷酸酶活性的影响。DHEAS 和雌二醇在体外暴露于这些分离株中不会诱导腺苷一磷酸（AMP）水解的任何变化。在用 DHEAS（0.01-1.0 μM）处理 2 小时后，DHEAS 处理寄生虫可抑制 VP60 分离株中 AMP 的水解，而在存在雌二醇时核苷酸水解没有明显变化。DHEAS 和雌二醇（0.01-1.0 μM）处理 2 小时可抑制 30236 分离株中 AMP 的水解。用 0.1 μM DHEAS 处理 12 小时可抑制 AMP 水解，而雌二醇不改变 VP60 分离株中核苷酸的水解。我们的研究结果表明，甾体激素及其受体对阴道滴虫的复杂影响可能会促进在暴露于这些激素时ecto-5'-核苷酸酶活性的变化。
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