Programa de Pós-Graduação em Biologia Celular e Molecular, Laboratório de Neuroquímica e Psicofarmacologia, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Avenida Ipiranga 6681, Porto Alegre, RS, Brazil.
Exp Parasitol. 2010 Jul;125(3):187-95. doi: 10.1016/j.exppara.2010.01.029. Epub 2010 Feb 14.
We investigated the effect of dehydroepiandrosterone sulfate (DHEAS) and 17beta-estradiol on NTPDase activity in fresh clinical (VP60) and long-term-grown (30236 ATCC) isolates of Trichomonas vaginalis followed by NTPDase gene transcriptional analysis. ATP hydrolysis was activated in vitro by 17beta-estradiol (0.01-1.0microM) in the VP60 isolate. Treatment for 2h with 17beta-estradiol (0.01-1microM) promoted an inhibition in nucleotide hydrolysis in the 30236 isolate whereas the 12h-treatment promoted an activation of nucleotide hydrolysis in both isolates. ADP hydrolysis was inhibited in vitro by 1.0-5.0microM DHEAS in the ATCC isolate. The treatment with DHEAS (0.01-1.0microM) for 2h inhibited ATP and ADP hydrolysis in VP60; however, during a 12h-treatment with DHEAS, nucleotide hydrolysis was inhibited in both isolates. Two NTPDase orthologous (NTPDaseA and NTPDaseB) were identified and the treatment with DHEAS for 12h was able to inhibit mRNA NTPDaseA transcript levels from the VP60. These findings demonstrate that NTPDase activity and gene expression pattern are modulated by exposure to steroids in T. vaginalis.
我们研究了硫酸去氢表雄酮（DHEAS）和 17β-雌二醇对新鲜临床（VP60）和长期培养（30236 ATCC）阴道毛滴虫分离株 NTPDase 活性的影响，随后进行了 NTPDase 基因转录分析。在 VP60 分离株中，17β-雌二醇（0.01-1.0μM）在体外激活了 ATP 水解。用 17β-雌二醇（0.01-1μM）处理 2 小时可促进 30236 分离株核苷酸水解的抑制，而 12 小时处理可促进两种分离株核苷酸水解的激活。在 ATCC 分离株中，1.0-5.0μM DHEAS 在体外抑制 ADP 水解。用 DHEAS（0.01-1.0μM）处理 2 小时可抑制 VP60 中的 ATP 和 ADP 水解；然而，用 DHEAS 处理 12 小时可抑制两种分离株中的核苷酸水解。鉴定了两个 NTPDase 同源物（NTPDaseA 和 NTPDaseB），用 DHEAS 处理 12 小时可抑制 VP60 中 NTPDaseA 转录本的 mRNA 水平。这些发现表明，NTPDase 活性和基因表达模式受阴道毛滴虫中类固醇暴露的调节。
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