Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
J Mol Neurosci. 2009 Nov;39(3):354-9. doi: 10.1007/s12031-009-9288-2.
Synaptic vesicle protein 2A (SV2A) involvement has been reported in the animal models of epilepsy. The aim of this study was to investigate the expression of SV2A in human intractable epilepsy (IE) brain tissue. Using immunohistochemistry, immunofluorescence, and Western blot, we detected SV2A expression in tissue samples from the anterior temporal neocortex of 33 patients who had been surgically treated for IE. We compared these tissues with nine histologically normal anterior temporal lobe samples from controls. SV2A immunoreactive staining was 0.1651+/-0.0564 in patient group and 0.2347+/-0.0187 in the control group (p<0.05) using immunohistochemistry, and this finding was consistently observed with Western blot analysis (0.1727 +/- 0.0471 versus 0.3976+/-0.0983, p<0.05). Immunofluorescence staining showed that SV2A was mainly accumulated in neurons. Our findings demonstrate that down-regulation of SV2A is present in patients with temporal lobe epilepsy.
突触小泡蛋白 2A（SV2A）参与了癫痫动物模型的研究。本研究旨在探讨 SV2A 在人类难治性癫痫（IE）脑组织中的表达。通过免疫组织化学、免疫荧光和 Western blot，我们检测了 33 例因 IE 接受手术治疗的患者前颞叶皮质组织样本中的 SV2A 表达情况。我们将这些组织与来自对照组的 9 例组织学正常的前颞叶样本进行了比较。免疫组织化学检测结果显示，患者组 SV2A 免疫反应性染色为 0.1651+/-0.0564，对照组为 0.2347+/-0.0187（p<0.05），Western blot 分析结果也一致（0.1727 +/- 0.0471 与 0.3976+/-0.0983，p<0.05）。免疫荧光染色显示 SV2A 主要在神经元中积累。我们的研究结果表明，颞叶癫痫患者存在 SV2A 的下调。
