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拟南芥 halleri × 拟南芥 lyrata 杂种后代中锌的分布和形态及其对锌的不同积累能力。

Zinc distribution and speciation in Arabidopsis halleri x Arabidopsis lyrata progenies presenting various zinc accumulation capacities.

机构信息

Environmental Geochemistry Group, LGIT, University J. Fourier and CNRS, BP 53, 38041 Grenoble Cedex 9, France.

Laboratoire de Génétique et Evolution des Populations Végétales, UMR 8016, CNRS, Université Lille 1, F-59655 Villeneuve d'Ascq, France.

出版信息

New Phytol. 2009 Nov;184(3):581-595. doi: 10.1111/j.1469-8137.2009.02996.x. Epub 2009 Sep 15.

Abstract

The purpose of this study was to investigate the relationship between the chemical form and localization of zinc (Zn) in plant leaves and their Zn accumulation capacity. An interspecific cross between Arabidopsis halleri sp. halleri and Arabidopsis lyrata sp. petrea segregating for Zn accumulation was used. Zinc (Zn) speciation and Zn distribution in the leaves of the parent plants and of selected F(1) and F(2) progenies were investigated by spectroscopic and microscopic techniques and chemical analyses. A correlation was observed between the proportion of Zn being in octahedral coordination complexed to organic acids and free in solution (Zn-OAs + Zn(aq)) and Zn content in the leaves. This pool varied between 40% and 80% of total leaf Zn depending on the plant studied. Elemental mapping of the leaves revealed different Zn partitioning between the veins and the leaf tissue. The vein : tissue fluorescence ratio was negatively correlated with Zn accumulation. The higher proportion of Zn-OAs + Zn(aq) and the depletion of the veins in the stronger accumulators are attributed to a higher xylem unloading and vacuolar sequestration in the leaf cells. Elemental distributions in the trichomes were also investigated, and results support the role of carboxyl and/or hydroxyl groups as major Zn ligands in these cells.

摘要

本研究旨在探讨植物叶片中锌(Zn)的化学形态和定位与其积累能力之间的关系。使用拟南芥 halleri sp. halleri 和拟南芥 lyrata sp. petrea 的种间杂交,其对 Zn 的积累能力呈分离状态。采用光谱和显微镜技术以及化学分析方法研究了亲本植物以及选定的 F1 和 F2 后代叶片中 Zn 的形态和分布。结果发现,Zn 与有机酸形成八面体配位复合物并以游离形式存在于溶液中的比例(Zn-OAs + Zn(aq))与叶片中的 Zn 含量呈正相关。这个部分在不同的植物中占总叶 Zn 的 40%到 80%不等。叶片的元素映射揭示了叶脉和叶组织之间不同的 Zn 分配。叶脉与组织荧光比值与 Zn 积累呈负相关。较强的积累者中 Zn-OAs + Zn(aq) 的比例较高,且叶脉含量较低,这归因于更高的木质部卸载和叶细胞中的液泡螯合。还研究了毛状体中的元素分布,结果支持了羧基和/或羟基作为这些细胞中主要 Zn 配体的作用。

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