Programa de Pós-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande Do Sul, Porto Alegre, Brazil.
Departamento de Botânica, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, Porto Alegre, RS, 9500, Brazil.
Sci Rep. 2021 Mar 5;11(1):5278. doi: 10.1038/s41598-021-84508-y.
Zinc (Zn) is a key micronutrient for plants and animals, and understanding Zn homeostasis in plants can improve both agriculture and human health. While root Zn transporters in plant model species have been characterized in detail, comparatively little is known about shoot processes controlling Zn concentrations and spatial distribution. Previous work showed that Zn hyperaccumulator species such as Arabidopsis halleri accumulate Zn and other metals in leaf trichomes. To date there is no systematic study regarding Zn accumulation in the trichomes of the non-accumulating, genetic model species A. thaliana. Here, we used Synchrotron X-Ray Fluorescence mapping to show that Zn accumulates at the base of trichomes of A. thaliana. Using transgenic and natural accessions of A thaliana that vary in bulk leaf Zn concentration, we demonstrate that higher leaf Zn increases total Zn found at the base of trichome cells. Our data indicates that Zn accumulation in trichomes is a function of the Zn status of the plant, and provides the basis for future studies on a genetically tractable plant species to understand the molecular steps involved in Zn spatial distribution in leaves.
锌（Zn）是动植物的关键微量营养素，了解植物中的锌稳态可以改善农业和人类健康。虽然植物模式物种中的根锌转运蛋白已被详细描述，但关于控制锌浓度和空间分布的茎部过程知之甚少。以前的工作表明，拟南芥等锌超积累物种在叶毛状体中积累锌和其他金属。迄今为止，关于非积累遗传模式物种拟南芥毛状体中的锌积累还没有系统的研究。在这里，我们使用同步加速器 X 射线荧光映射来显示锌在拟南芥毛状体的基部积累。我们使用转基因和天然拟南芥品系进行研究，这些品系的叶片整体锌浓度不同，结果表明，更高的叶片锌浓度会增加毛状体细胞基部的总锌含量。我们的数据表明，毛状体中的锌积累是植物锌状态的一个功能，为未来在遗传上可处理的植物物种中研究参与叶片中锌空间分布的分子步骤提供了基础。
