State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
Fish Shellfish Immunol. 2010 Jan;28(1):221-7. doi: 10.1016/j.fsi.2009.09.001. Epub 2009 Sep 15.
Complement component 1 inhibitor (C1INH) is a crucial protein in controlling activation of many plasma mediator pathways and can directly interact with Gram negative bacteria. The full-length cDNA of lycC1INH gene was identified from the large yellow croaker. It is of 2046 nucleotides (nt) encoding a protein of 599 amino acids, with a 5'-untranslated region of 99 nt and a 3'-untranslated region of 147 nt including the poly (A) tail. The deduced protein contains a C-terminal serpin (serine protease inhibitor) domain, and two N-terminus immunoglobulin domains without significant homology to other species. Western blot analysis of the protein expression showed that the expression of lycC1INH was obviously up-regulated in liver, spleen and head kidney of the fish challenged by attenuated live Vibrio anguillarum strain. This indicated that lycC1INH might be involved in the immune response of large yellow croaker to bacterial challenge.
补体成分 1 抑制剂（C1INH）是控制许多血浆介质途径激活的关键蛋白，可直接与革兰氏阴性菌相互作用。从大黄鱼中鉴定出全长 lycC1INH 基因 cDNA。它由 2046 个核苷酸（nt）编码，编码 599 个氨基酸的蛋白质，具有 5'非翻译区 99 个核苷酸和 3'非翻译区 147 个核苷酸，包括多聚（A）尾。推导的蛋白质包含 C 末端丝氨酸蛋白酶抑制剂（serine protease inhibitor）结构域和两个 N 端免疫球蛋白结构域，与其他物种没有明显的同源性。蛋白质表达的 Western blot 分析表明，在受到减毒活鳗弧菌菌株攻击的鱼的肝脏、脾脏和头肾中，lycC1INH 的表达明显上调。这表明 lycC1INH 可能参与大黄鱼对细菌攻击的免疫反应。
