Analytische Chemie-Elektroanalytik & Sensorik, Ruhr-Universität Bochum, Universitätstr. 150, 44780 Bochum, Germany.
Anal Bioanal Chem. 2009 Nov;395(6):1693-706. doi: 10.1007/s00216-009-3052-x.
Electrodeposition polymers can be precipitated on electrode surfaces upon electrochemical-induced modulations of the pH value in the diffusion zone in front of the electrode. The formed polymer films can be used as immobilization matrices in amperometric biosensors. In order to rationally control the thus obtained biosensor properties, it is indispensable to develop strategies for the reproducible synthesis of electrodeposition polymers as well as methods for the non-manual and reproducible sensor fabrication. Based on instrumental developments such as a specifically designed parallel synthesizer with improved stirring and temperature control, an automatic pipetting robot for the preparation of the monomer mixtures and controlled removal of polymerization inhibitors, the reproducible synthesis of libraries of electrodeposition polymers was achieved. Moreover, the polymerization process could be monitored using in-line thermocouples, and it could be shown that the chosen strategies led to reproducible polymerization reactions. By adaptation of an electrochemical robotic system integrating a Au microtiter plate and automatic electrode cleaning by means of a polishing wheel reproducible biosensor fabrication using glucose oxidase as a model enzyme could be demonstrated. These results open the route for the rational development of biosensors and control of the sensor properties by choosing specifically designed electrodeposition polymers.
电沉积聚合物可以在电极表面上沉淀，方法是在电极前的扩散区中电化学诱导 pH 值的调制。形成的聚合物膜可用作电流型生物传感器中的固定化基质。为了合理控制由此获得的生物传感器性能，有必要开发用于电沉积聚合物的可重现合成的策略，以及用于非手动和可重现的传感器制造的方法。基于仪器的发展，例如具有改进的搅拌和温度控制的专门设计的平行合成器、用于制备单体混合物的自动移液机器人以及聚合抑制剂的受控去除，实现了电沉积聚合物库的可重现合成。此外，还可以使用在线热电偶监测聚合过程，并表明所选择的策略导致了可重现的聚合反应。通过集成 Au 微滴定板的电化学机器人系统的适应以及通过抛光轮进行自动电极清洁，可以证明使用葡萄糖氧化酶作为模型酶进行可重现的生物传感器制造。这些结果为通过选择专门设计的电沉积聚合物来合理开发生物传感器和控制传感器性能开辟了道路。
