Department of Trauma Surgery, Plastic and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany.
Planta Med. 2010 Feb;76(3):235-40. doi: 10.1055/s-0029-1186136. Epub 2009 Sep 17.
The isoflavonoids found in soy have attracted great interest as dietary phytoestrogens that might be effective for postmenopausal hormone replacement therapy. Special attention has been devoted to the hormonal effects of various isoflavonoids, like genistein (GEN) and daidzein's (DAID) potent metabolite, equol (EQ). Here we aimed to investigate the short-term effects of genistein and equol on the proximal femur of ovariectomized (OVX) rats. Forty-eight, 3-month-old female Sprague-Dawley rats were ovarectomized; after eight weeks the bilateral osteotomy and osteosynthesis (OS) of their tibiae was performed and the rats were randomly divided into the following four groups: OVX control group (C), treated with estradiol-17beta (E2) -benzoate (E; daily intake 0.086 mg/d per animal), genistein (GEN; daily intake 12.7 mg/d per animal) and equol (EQ; daily intake 4.65 mg/d per animal). At 5 weeks postoperatively (OS), the breaking test was performed on the trochanteric region of femur. Additionally, histomorphometric assessment, and trabecular and cortical bone microstructure analyses were performed. The relative gain of body weight (BW) in the EQ (24 %) group was significantly (p < 0.05) lower than in the C (33 %) and GEN (30 %) groups. After treatment for 5 weeks, the maximal load (F(max)) and yield load (yL) were higher (p < 0.05 for the weight-adapted results) in the E (188.4 N resp. 113.1 N) and EQ (177.3 N resp. 112 N) groups as compared to C (162.8 N resp. 109.1 N) and GEN (165.7 N resp. 108.8 N). In the histomorphometric tests the E- (trabecular area (Tb.Ar) = 74.93 %, trabecular nodes/mm(2) (N.Nd/mm(2)) = 48.65) and EQ-treated (Tb.Ar = 63.13 %, N.Nd/mm(2) = 43.72) animals showed significant improvement with regard to Tb.Ar and trabecular connectivity (N.Nd./mm(2)) in comparison to C (Tb.Ar = 46.84, N.Nd/mm(2) = 31.86) and GEN (Tb.Ar = 48.22 %, N.Nd/mm(2) = 34.15). There were no differences in relative cortical width (Ct.Wi) among the four groups. The treatment with EQ resulted in improved biomechanical and histomorphometric properties as compared to the treatment with GEN. Thus, of the studied substances, EQ seems to be a possible alternative to hormone replacement therapy, but further studies are needed.
大豆中的异黄酮作为膳食植物雌激素引起了极大的兴趣，可能对绝经后激素替代疗法有效。人们特别关注各种异黄酮的激素作用，如染料木黄酮（GEN）和大豆苷元（DAID）的有效代谢物，雌马酚（EQ）。在这里，我们旨在研究染料木黄酮和雌马酚对去卵巢（OVX）大鼠股骨近端的短期影响。将 48 只 3 个月大的雌性 Sprague-Dawley 大鼠去卵巢；八周后，对其胫骨进行双侧截骨和内固定（OS），并将大鼠随机分为以下四组：OVX 对照组（C），用苯甲酸雌二醇-17β（E2）治疗（每天每只动物摄入 0.086mg/d），染料木黄酮（GEN）治疗（每天每只动物摄入 12.7mg/d）和雌马酚（EQ）治疗（每天每只动物摄入 4.65mg/d）。在术后 5 周（OS）时，对股骨转子区进行断裂试验。此外，还进行了组织形态计量评估以及小梁和皮质骨微观结构分析。EQ（24%）组的体重相对增加（BW）明显（p<0.05）低于 C（33%）和 GEN（30%）组。治疗 5 周后，E 组（188.4N 分别为 113.1N）和 EQ 组（177.3N 分别为 112N）的最大负荷（Fmax）和屈服负荷（yL）高于 C 组（162.8N 分别为 109.1N）和 GEN 组（165.7N 分别为 108.8N）（重量适应结果）。在组织形态计量学测试中，E 治疗（小梁面积（Tb.Ar）=74.93%，小梁节点/mm²（N.Nd/mm²）=48.65）和 EQ 治疗（Tb.Ar=63.13%，N.Nd/mm²=43.72%）的动物与 C 组（Tb.Ar=46.84%，N.Nd/mm²=31.86%）和 GEN 组（Tb.Ar=48.22%，N.Nd/mm²=34.15%）相比，Tb.Ar 和小梁连接性（N.Nd/mm²）显著改善。四组间相对皮质宽度（Ct.Wi）无差异。与 GEN 治疗相比，EQ 治疗可改善生物力学和组织形态计量学特性。因此，与研究中的其他物质相比，EQ 似乎是激素替代疗法的一种可能替代物，但仍需要进一步研究。
