Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, Gdansk, Poland.
J Sep Sci. 2009 Oct;32(20):3539-43. doi: 10.1002/jssc.200900297.
We are presenting the application of CE technique with dual-channel LIF detection for the simultaneous separation of DNA fragments labeled with two different fluorescence dyes. The optimal conditions of the analysis were determined for the separation of amplified fragment length polymorphism (AFLP) fragments labeled with 5'-6-carboxyfluorescein (6-FAM) and the DNA size standard labeled with sulfoindocyanine succinimidyl ester (Cy-5). CE equipped with both argon ion and diode lasers is a good alternative for sequencers and might be applied in analyses of PCR products generated by various fingerprinting methods.
我们介绍了双通道 LIF 检测 CE 技术在同时分离两种不同荧光染料标记的 DNA 片段中的应用。为了分离用 5'-6-羧基荧光素（6-FAM）标记的扩增片段长度多态性（AFLP）片段和用磺基吲哚氰基琥珀酰亚胺酯（Cy-5）标记的 DNA 分子量标准，确定了最佳分析条件。配备氩离子和二极管激光器的 CE 是测序仪的良好替代品，可应用于各种指纹图谱方法生成的 PCR 产物分析。
