[胶质母细胞瘤患者脑内水通道蛋白4表达及其与脑水肿的关系]
[AQP4 expression in the brains of patients with glioblastoma and its association with brain edema].
作者信息
Mou Ke-Jie, Mao Qing, Chen Mi-Na, Xia Xiao-Qiang, Ni Ren-Yong, Wang Peng, Liu Yan-Hui
机构信息
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Jul;40(4):651-4.
OBJECTIVE
To investigate the expression of aquaporin-4 (AQP4) in the brains of patients with glioblastoma and its association with brain edema.
METHODS
Immunofluorescence cytochemistry and western blot tests were performed to detect the expression of AQP4 in the brain tumors and the adjacent tissues in 30 patients with glioblastoma. The association between AQP4 and the extent of brain edema was analysed.
RESULTS
The AQP4 immunoreactive cells were mainly astrocytes in the brains, which were extensively distributed in the intracytoplasm. Higher expressions of AQP4 were found in the brain tumors and adjacent tissues in the patients with glioblastoma than in the normal controls (P<0.05). More AQP4 were distributed in the tumor adjacent tissues than in the tumors (P<0.05). The AQP4 was positively correlated with the extent of brain edema.
CONCLUSION
AQP4 overexpress in the brain tumors and adjacent tissues, which is associated with the extent of brain edema. Cytotoxic and vasogenic edemas may coexist in the cerebral edema induced by glioblastoma.
目的
探讨水通道蛋白4（AQP4）在胶质母细胞瘤患者脑组织中的表达及其与脑水肿的关系。
方法
对30例胶质母细胞瘤患者的脑肿瘤及邻近组织进行免疫荧光细胞化学和蛋白质印迹检测，以检测AQP4的表达。分析AQP4与脑水肿程度之间的关系。
结果
脑中AQP4免疫反应性细胞主要为星形胶质细胞，广泛分布于胞质内。胶质母细胞瘤患者脑肿瘤及邻近组织中AQP4的表达高于正常对照组（P<0.05）。肿瘤邻近组织中AQP4的分布多于肿瘤组织（P<0.05）。AQP4与脑水肿程度呈正相关。
结论
AQP4在脑肿瘤及邻近组织中过表达，这与脑水肿程度相关。胶质母细胞瘤所致脑水肿可能同时存在细胞毒性水肿和血管源性水肿。
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