Liu Shu-Kun, Wangh Ling, Chen Chen, Zhang Zun-Zhen
Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 61004, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Jul;40(4):667-71.
To determine the oxidative damage of mainstream smoke (MS) on Human bronchial epithelial cells (HBE) and its role in lung cancer.
MTT assay was used to test the cytotoxicity of MS on HBE. The HBE cells were treated with different concentrations of MS for 12 h. The chromatosome damage and DNA strand breaks were measured by micronucleus test and alkaline comet assay respectively. The contents of ROS in the HBE cells were determined using fluorescence method.
With the increase of MS, the viability of HBE cells decreased. The IC50 decreased with the increasing exposure time to MS, showing significant dose-effect and time-effect relationships. The MS induced DNA strand break in the HBE cells. The comet, L Tail, Tail DNA and OTM increased with the increase of MS concentrations. Cigarette smoke also induced chromosome damage. The micronucleus rate of the HBE cells exposed to more than one cigarette/L of MS was significantly greater than the controls (P<0.05). The ROS increased with the concentration of MS.
MS induces ROS in HBE cells, resulting in increased cytotoxicity, chromosome damage and DNA strand breaks, which suggests that oxidative damage is an important mechanism of lung cancer caused by MS.
确定主流烟雾（MS）对人支气管上皮细胞（HBE）的氧化损伤及其在肺癌中的作用。
采用MTT法检测MS对HBE的细胞毒性。将HBE细胞用不同浓度的MS处理12小时。分别通过微核试验和碱性彗星试验检测染色体损伤和DNA链断裂情况。采用荧光法测定HBE细胞中活性氧（ROS）的含量。
随着MS浓度增加，HBE细胞活力下降。IC50随MS暴露时间延长而降低，呈现显著的剂量效应和时间效应关系。MS诱导HBE细胞DNA链断裂。彗星尾长、L尾、尾DNA和尾矩随MS浓度增加而增加。香烟烟雾还诱导染色体损伤。暴露于浓度超过1支/升MS的HBE细胞微核率显著高于对照组（P<0.05）。ROS随MS浓度增加而升高。
MS诱导HBE细胞产生ROS，导致细胞毒性增加、染色体损伤和DNA链断裂，提示氧化损伤是MS致肺癌的重要机制。
