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[稗草颖果中一种新型脂质转移蛋白的纯化与鉴定]

[The purification and characterization of a novel lipid transfer protein from caryopsis of barnyard grass (Echinochloa crusgalli)].

作者信息

Rogozhin E A, Odintsova T I, Musoliamov A Kh, Smirnov A N, Babakov A V, Egorov Ts A, Grishin E V

出版信息

Prikl Biokhim Mikrobiol. 2009 Jul-Aug;45(4):403-9.

PMID:19764607
Abstract

A novel lipid transfer protein called Ec-LTP was isolated from resting caryopsis of weed barnyard grass Echinochloa crusgalli (L.) Beauv.; its molecular weight, amino acid content and N-terminal amino acid sequence were determined. Ec-LTP was a 9150 Da protein, containing eight cysteine residues, which formed four disulfide bonds. The isolated protein could significantly inhibit the development of pathogenic fungi Phytophthora infestans and Helminthosporium sativum, causing the late blight of potato and tomato and the root rot of herbs, respectively.

摘要

从杂草稗草(Echinochloa crusgalli (L.) Beauv.)的休眠颖果中分离出一种名为Ec-LTP的新型脂质转移蛋白;测定了其分子量、氨基酸含量和N端氨基酸序列。Ec-LTP是一种9150 Da的蛋白质,含有八个半胱氨酸残基,形成四个二硫键。分离出的蛋白质能显著抑制致病真菌致病疫霉(Phytophthora infestans)和小麦根腐病菌(Helminthosporium sativum)的生长,它们分别导致马铃薯和番茄晚疫病以及草本植物根腐病。

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引用本文的文献

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J Biol Chem. 2011 Jul 15;286(28):25145-53. doi: 10.1074/jbc.M110.200378. Epub 2011 May 11.