Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russian Federation.
Peptides. 2012 Nov;38(1):33-40. doi: 10.1016/j.peptides.2012.08.009. Epub 2012 Aug 23.
A number of defense polypeptides from latent seeds of weed cereal barnyard grass (Echinochloa crusgalli L.) has been isolated and characterized using an acidic extraction and high performance liquid chromatography methods in combination with MALDI-TOF mass spectrometry and Edman sequencing. Members of three antimicrobial peptide families and two protease inhibitor families were found to be localized in barnyard grass seeds. Their biological activity concerning to Gram-Positive and Gram-Negative phytopathogenic bacteria, as well as oomycete Phytophthora infestans, has been investigated. Diversity of barnyard grass defense peptides is a significant factor that provides a resistance of E. crusgalli seeds to germination and latent phases.
利用酸性提取和高效液相色谱法结合 MALDI-TOF 质谱和 Edman 测序，从杂草谷类稗草（Echinochloa crusgalli L.）的潜伏种子中分离和鉴定了多种防御多肽。发现三种抗菌肽家族和两种蛋白酶抑制剂家族的成员定位于稗草种子中。研究了它们对革兰氏阳性和革兰氏阴性植物病原菌以及卵菌 Phytophthora infestans 的生物活性。稗草防御肽的多样性是稗草种子对发芽和潜伏阶段具有抗性的重要因素。
