Il'ina A V, Zagorskaia D S, Levov A N, Albulov A I, Kovacheva N P, Varlamov V P
Prikl Biokhim Mikrobiol. 2009 Jul-Aug;45(4):415-21.
This article describes the optimal conditions for the enzymatic hydrolysis of chitosan and its chemically-modified derivatives using the preparation extracted from the king crab hepatopancrease possessing pronounced hydrolythic activity. The following preparations were used: chitosan with a molecular weight of 100 kDa and an acetylation level of 0.15, carboxymethyl chitosan 200 kDa witih an extent of replacement of 0.23, and N-succinyl chitosan 390 kDa with an extent of replacement of 0.8. Low molecular-weight samples of chitosan and of its modified derivatives were obtained with the yields of 85, 55, and 80%, respectively. The conditions of the hydrolysis were as follows: an enzyme: substrate ratio of 1:200, 37 degrees C, and 20 h duration of hydrolysis.
本文描述了使用从具有显著水解活性的帝王蟹肝胰腺中提取的制剂，对壳聚糖及其化学改性衍生物进行酶促水解的最佳条件。使用了以下制剂：分子量为100 kDa且乙酰化水平为0.15的壳聚糖、取代度为0.23的200 kDa羧甲基壳聚糖以及取代度为0.8的390 kDa N-琥珀酰壳聚糖。分别以85%、55%和80%的产率获得了壳聚糖及其改性衍生物的低分子量样品。水解条件如下：酶与底物的比例为1:200，温度为37℃，水解持续时间为20小时。
