Gritsaĭ O B, Dubynin V A, Bespalova Zh D, Pilipenko V E
Zh Evol Biokhim Fiziol. 2009 Jul-Aug;45(4):391-7.
The ability of several alimentary opioid peptides (exorphin C, rubiscolin-5, cytochrophi-4) and endorphins (met-enkephalin, dynotphin A(1-10), beta-neoendorphin) to change the escape reaction of the cockroaches Periplaneta americana at their placement into a hot chamber was studied. The ED50 values increasing twice the insect stay time in the hot chamber as well as duration and dynamics of the effects were determined. It has been shown that ED5 decreases statistically significantly with increase of the length of the peptide molecule and its affinity of duration of the effects and to an increase of their affinity to delta-receptors - to prolongation of the reaction (more than150 min). In the group of alimentary peptides (exorphins) the most active was a fragment of D-ribulose-1,5-biphosphate carboxylase/oxigenase rubiscolin-5 (ED5 = 386 nM per individual). This might indicate a specific ability of some plant proteins to regulate (decrease) the insect protective behavior.
研究了几种消化性阿片样肽（外啡肽C、核酮糖-1,5-二磷酸羧化酶/加氧酶5、细胞色素C-4）和内啡肽（甲硫氨酸脑啡肽、强啡肽A(1-10)、β-新内啡肽）对美洲大蠊置于热室时逃避反应的影响。测定了使昆虫在热室停留时间增加两倍的半数有效剂量（ED50）值以及效应的持续时间和动态变化。结果表明，随着肽分子长度的增加及其对效应持续时间的亲和力以及对δ受体亲和力的增加，ED5在统计学上显著降低——反应延长（超过150分钟）。在消化性肽（外啡肽）组中，最活跃的是核酮糖-1,5-二磷酸羧化酶/加氧酶的片段核酮糖-1,5-二磷酸羧化酶5（每只昆虫的ED5 = 386 nM）。这可能表明某些植物蛋白具有调节（降低）昆虫保护行为的特定能力。
