Pasinetti G M, Morgan D G, Johnson S A, Millar S L, Finch C E
Andrus Gerontology Center, University of Southern California, Los Angeles 90089-0191.
J Neurochem. 1990 Nov;55(5):1793-9. doi: 10.1111/j.1471-4159.1990.tb04970.x.
Tyrosine hydroxylase (TH)-mRNA, assayed by in situ hybridization combined with TH immunocytochemistry, showed a selective increase in the ventral tegmental area (A-10) but not in the substantia nigra (A-9) midbrain dopaminergic (DAergic) neurons 3 days after reserpine treatment. TH-mRNA in locus ceruleus noradrenergic (A-4) neurons was increased by reserpine, as confirmed by RNA blot hybridization. These findings show that TH-mRNA is differentially regulated in midbrain DAergic neurons in response to reserpine.
通过原位杂交结合酪氨酸羟化酶（TH）免疫细胞化学检测发现，利血平处理3天后，中脑多巴胺能（DAergic）神经元腹侧被盖区（A-10）的TH-mRNA有选择性增加，但黑质（A-9）中没有。RNA印迹杂交证实，利血平可使蓝斑去甲肾上腺素能（A-4）神经元中的TH-mRNA增加。这些发现表明，中脑DAergic神经元中的TH-mRNA在对利血平的反应中受到不同调节。
