Mascari T M, Foil L D
Department of Entomology, Louisiana State University Agricultural Center, Agricultural Experiment Station, 402 Life Sciences, Baton Rouge, LA 70803, USA.
J Med Entomol. 2009 Sep;46(5):1131-7. doi: 10.1603/033.046.0521.
The purpose of this study was to evaluate the use of rhodamine B as an orally delivered biomarker for rodents and a feed-through transtadial biomarker for phlebotomine sand flies (Diptera: Psychodidae). Rhodamine B-treated hamsters were visibly marked for up to 8 wk, and their feces were fluorescent when examined under a fluorescence microscope. The development and survival of sand fly larvae fed feces of rhodamine B-treated hamsters were not significantly different from control sand flies. Adult male and female sand flies, that had been fed as larvae the feces of rhodamine B-treated hamsters, were fluorescent when examined using fluorescent microscopy and could be distinguished from control sand flies. Adult female sand flies that took bloodmeals from rhodamine B-treated hamsters were fluorescent when examined immediately after feeding. Rhodamine B incorporated rodent baits could be used to detect adult male and female sand flies that fed on the feces of baited rodents as larvae, or adult female sand flies that have taken a bloodmeal from bait-fed rodents. This would allow the delineation of specific foci with rodent-sand fly associations that would be susceptible to control by using feed-through or systemic insecticides.
本研究的目的是评估若丹明B作为啮齿动物口服生物标志物以及白蛉（双翅目：毛蠓科）经口传递的跨龄期生物标志物的用途。用若丹明B处理过的仓鼠在长达8周的时间内都有明显的标记，并且在荧光显微镜下检查时其粪便呈荧光状。取食若丹明B处理过的仓鼠粪便的白蛉幼虫的发育和存活情况与对照白蛉没有显著差异。幼虫取食若丹明B处理过的仓鼠粪便的成年雌雄白蛉，在使用荧光显微镜检查时呈荧光状，并且可以与对照白蛉区分开来。取食若丹明B处理过的仓鼠血液的成年雌白蛉在取食后立即检查时呈荧光状。掺入啮齿动物诱饵中的若丹明B可用于检测幼虫取食诱饵啮齿动物粪便的成年雌雄白蛉，或取食诱饵啮齿动物血液的成年雌白蛉。这将有助于确定存在啮齿动物 - 白蛉关联的特定疫源地，这些疫源地可通过经口传递或内吸性杀虫剂进行控制。
