Kim Kyeong Soon, Tsuda Yoshio, Yamada Akio
Department of Medical Entomology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan.
J Med Entomol. 2009 Sep;46(5):1230-4. doi: 10.1603/033.046.0535.
Bloodmeal identification and the detection of avian malaria parasite from mosquitoes (Diptera: Culicidae) were carried out by polymerase chain reaction-based methods for field samples collected in coastal areas of Tokyo Bay, Japan, from April to October 2007. The following seven mosquito species were collected: Aedes albopictus (Skuse), Culex pipiens pallens Coquillett, Culex pipiens form molestus Forskal, Culex tritaeniorhynchus Giles, Culex inatomii Kamimura & Wada, Culex bitaeniorhynchus Giles, and Lutzia vorax Edwards. Forty blood-fed mosquitoes were collected and 95% of bloodmeals of Cx. pipiens pallens were avian-derived, whereas only mammalian bloodmeals were identified for Ae. albopictus. Plasmodium DNA was amplified from 65% (15/23) of blood-fed Cx. pipiens pallens and unfed females of Cx. pipiens pallens and Cx. pipiens form molestus with a minimum infection rate of 29.9 and 13.5, respectively. One unfed female of Lt. vorax was also positive for Plasmodium parasites. Five genetically distinct lineages of Plasmodium were identified, with 0.21 to 5.86% sequence divergence. Rinshi-8, the most prevalent lineage at our study site, was identical to the published sequence of Plasmodium relictum-P5.
2007年4月至10月，在日本东京湾沿海地区采集野外样本，采用基于聚合酶链反应的方法对蚊子（双翅目：蚊科）的血餐进行鉴定并检测禽疟原虫。共采集到以下七种蚊子：白纹伊蚊（Skuse）、淡色库蚊（Coquillett）、骚扰库蚊（Forskal）、三带喙库蚊（Giles）、稻叶库蚊（Kamimura & Wada）、二带喙库蚊（Giles）和凶猛卢氏蚊（Edwards）。采集到40只吸食血液的蚊子，淡色库蚊95%的血餐来源于禽类，而白纹伊蚊仅鉴定出哺乳动物血餐。从65%（15/23）吸食血液的淡色库蚊以及淡色库蚊和骚扰库蚊的未吸血雌蚊中扩增出疟原虫DNA，其最低感染率分别为29.9和13.5。一只未吸血的凶猛卢氏蚊雌蚊疟原虫检测也呈阳性。鉴定出五种基因不同的疟原虫谱系，序列差异为0.21%至5.86%。Rinshi - 8是我们研究地点最常见的谱系，与已发表的残疟原虫 - P5序列相同。
