Orion Pharma, Department of Oncology and Critical Care Research, Turku, Finland.
Horm Behav. 2010 Jul;58(2):341-51. doi: 10.1016/j.yhbeh.2009.08.011. Epub 2009 Sep 19.
In Experiment 1 castrated male rats were implanted with a Silastic capsule containing either E or cholesterol (CHOL) 35 days after castration. They were then tested for sexual incentive motivation and copulatory behaviors every 5th day for 3 weeks. None of the treatments affected sexual incentive motivation. After the last test, all subjects were implanted with DHT-containing Silastic capsules, and tests continued for another 3 weeks. While E+DHT enhanced sexual incentive motivation and copulatory behavior, DHT alone failed to do so. In Experiment 2 the aromatase inhibitor fadrozole (F) was combined with testosterone (T). T restored all behaviors to the level seen in intact rats, and F significantly reduced these effects. In fact, T+F was not different from DHT. T and DHT restored the weight of the prostate and seminal vesicles to levels close to those of intact rats. In Experiment 3 a lower dose of E was employed. Also this dose of E failed to affect sexual incentive motivation while E+DHT restored it to the level of intact animals. Castration enhanced the serum concentrations of LH and FSH. E alone caused a marked reduction, and E+DHT brought both gonadotropins back to the level of intact animals. It was concluded that the doses of E and DHT employed in these experiments were within or close to the physiological range, and that such doses of E completely fail to enhance sexual incentive motivation in castrated animals. DHT has small or no effects. It appears that sexual incentive motivation and copulation require simultaneous stimulation of androgen and estrogen receptors.
在实验 1 中，去势雄性大鼠在去势 35 天后被植入含有 E 或胆固醇 (CHOL) 的硅酮胶囊。然后，他们每隔 5 天进行一次性激励动机和交配行为测试，持续 3 周。这些处理都没有影响性激励动机。最后一次测试后，所有受试者都被植入含有 DHT 的硅酮胶囊，测试又持续了 3 周。虽然 E+DHT 增强了性激励动机和交配行为，但 DHT 单独作用却没有。在实验 2 中，芳香酶抑制剂 fadrozole (F) 与睾酮 (T) 结合使用。T 将所有行为恢复到完整大鼠的水平，F 显著降低了这些效果。事实上，T+F 与 DHT 没有区别。T 和 DHT 将前列腺和精囊的重量恢复到接近完整大鼠的水平。在实验 3 中，使用了较低剂量的 E。同样，这种剂量的 E 也未能影响性激励动机，而 E+DHT 将其恢复到完整动物的水平。去势增强了血清中 LH 和 FSH 的浓度。E 单独作用会导致明显降低，而 E+DHT 则将两种促性腺激素恢复到完整动物的水平。结论是，这些实验中使用的 E 和 DHT 剂量在生理范围内或接近生理范围，而这种剂量的 E 完全不能增强去势动物的性激励动机。DHT 作用很小或没有。似乎性激励动机和交配需要同时刺激雄激素和雌激素受体。
