Center for Neutron Research, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899-6102, United States.
Int J Biol Macromol. 2009 Dec 1;45(5):532-4. doi: 10.1016/j.ijbiomac.2009.09.002. Epub 2009 Sep 19.
DNA undergoes a helix-to-coil transition (also called denaturation transition) upon heating. This transition can also be facilitated by using solvent mixtures (for example water-alcohol). An increase in the hydrophobic tail of the second solvent molecule first decreases then increases the melting temperature appreciably. Measurement on 4% DNA in a series of water-alcohol mixtures shows that the helix-to-coil melting transition is driven by the solvent ability to cross the hydrophobic sugar-rich region. DNA is behaving like a cylindrical micelle.
DNA 在受热时会发生螺旋到线圈的转变（也称为变性转变）。这种转变也可以通过使用溶剂混合物（例如水-醇）来促进。第二溶剂分子的疏尾增加首先会显著降低然后增加熔点。对一系列水-醇混合物中 4%的 DNA 的测量表明，螺旋到线圈的熔化转变是由溶剂穿过富含疏水糖的区域的能力驱动的。DNA 的行为就像一个圆柱形胶束。
