Farber S M, Alekseev V V, Kostiukovskiĭ V M, Zykin L F, Golubinskiĭ E P
Zh Mikrobiol Epidemiol Immunobiol. 1977 Jul(7):81-5.
Spheroplasts were obtained from the causative agents of glanders and melioidosis under the effect of lysozyme and antibiotics. In the capacity of an inducing agent lysozyme was effective in high concentration only (0.4%); preliminary washing and incubation in sucrose were necessary to obtain glanders spheroplasts. Of the antibiotics studied penicillin was more useful for obtaining melioidosis spheroplasts and ampicillin--for glanders spheroplasts. Membrane preparations were derived from the spheroplasts of glanders and melioidosis causative agents.
在溶菌酶和抗生素的作用下，从鼻疽和类鼻疽的病原体中获得了原生质球。作为诱导剂，溶菌酶仅在高浓度（0.4%）时有效；为了获得鼻疽原生质球，需要进行初步洗涤并在蔗糖中孵育。在所研究的抗生素中，青霉素对获得类鼻疽原生质球更有用，氨苄青霉素对鼻疽原生质球更有用。膜制剂来自鼻疽和类鼻疽病原体的原生质球。
