Ecosystems Programme, Biosystems Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark, Roskilde, Denmark.
Plant Biol (Stuttg). 2009 Nov;11 Suppl 1:43-8. doi: 10.1111/j.1438-8677.2009.00202.x.
This study examines the effects of different irradiance types on aerobic methane (CH(4)) efflux rates from terrestrial plant material. Furthermore, the role of the enzyme pectin methyl esterase (PME) on CH(4) efflux potential was also examined. Different types of plant tissue and purified pectin were incubated in glass vials with different combinations of irradiation and/or temperature. Purified dry pectin was incubated in solution, and with or without PME. Before and after incubation, the concentration of CH(4) was measured with a gas chromatograph. Rates of CH(4) emission were found to depend exponentially on temperature and linearly on UV-B irradiance. UV-B had a greater stimulating effect than UV-A, while visible light had no effect on emission rates. PME was found to substantially reduce the potential for aerobic CH(4) emissions upon demethylation of pectin.
本研究考察了不同光照类型对陆地植物材料有氧甲烷（CH(4)）排放速率的影响。此外，还研究了果胶甲酯酶（PME）对 CH(4)排放潜力的作用。将不同类型的植物组织和纯化的果胶在带有不同光照和/或温度组合的玻璃小瓶中孵育。将纯化的干果胶在溶液中孵育，并加入或不加入 PME。孵育前后，使用气相色谱仪测量 CH(4)的浓度。CH(4)排放速率发现与温度呈指数关系，与 UV-B 辐照度呈线性关系。UV-B 的刺激作用大于 UV-A，而可见光对排放速率没有影响。发现果胶去甲基化后，PME 大大降低了有氧 CH(4)排放的潜力。
