Chen Y M, Gomez-Lucia E, Okayama A, Tachibana N, Lee T H, Mueller N, Essex M
Department of Cancer Biology, Harvard School of Public Health, Boston, Massachusetts 02115.
Lancet. 1990 Nov 17;336(8725):1214-6. doi: 10.1016/0140-6736(90)92833-4.
To define the antibody profile of early seroconversion in infection with human T-cell lymphotropic virus type I (HTLV-I), consecutive serum samples from 10 subjects presumed to have seroconverted on the basis of the particle agglutination test were studied by three enzyme immunoassays and two confirmatory tests (radioimmunoprecipitation and western blot). 3 samples positive and 1 sample indeterminate in the confirmatory tests were reactive in one enzyme immunoassay, which used recombinant envelope antigen, but not in the other two enzyme immunoassays. 2 of 38 particle-agglutination-negative samples had a prozone effect. The confirmatory tests identified 8 seroconverters (7 women, 1 man); their serum samples were used to study the antibody reactivity by western blot assays to HTLV-I specific antigens (three recombinant proteins spanning the N-terminal, middle, and C-terminal env glycoprotein gp46; a recombinant transmembrane protein gp21; a recombinant tax protein; and three gag proteins [p28, p24, and p19]). All 8 seroconverters had antibody reactivities to the C-terminal region (aminoacid residues 229-308) of gp46 and to gag p19 and p24 when their seroconversion was detected.
为确定人类嗜T淋巴细胞病毒I型（HTLV-I）感染早期血清转化的抗体谱，对10名基于颗粒凝集试验推测已发生血清转化的受试者的连续血清样本进行了三项酶免疫测定和两项确证试验（放射免疫沉淀和免疫印迹）研究。在确证试验中呈阳性的3份样本和1份不确定样本在一项使用重组包膜抗原的酶免疫测定中呈反应性，但在其他两项酶免疫测定中无反应性。38份颗粒凝集试验阴性样本中有2份出现前带效应。确证试验鉴定出8名血清转化者（7名女性，1名男性）；他们的血清样本用于通过免疫印迹试验研究针对HTLV-I特异性抗原（三种跨越N端、中间和C端env糖蛋白gp46的重组蛋白；一种重组跨膜蛋白gp21；一种重组tax蛋白；以及三种gag蛋白[p28、p24和p19]）的抗体反应性。在检测到血清转化时，所有8名血清转化者对gp46的C端区域（氨基酸残基229 - 308）以及gag p19和p24均有抗体反应性。
