Department of Immunohaematology, Institute of Haematology and Transfusion Medicine, Warsaw, Poland.
J Viral Hepat. 2010 Jun;17(6):444-52. doi: 10.1111/j.1365-2893.2009.01192.x. Epub 2009 Sep 25.
Hepatitis B virus (HBV) genotypes have distinct geographical distributions and influence severity of clinical outcome and response to antiviral therapies. HBV polymorphism in HBV surface antigen (HBsAg) positive first time blood donors from Poland was examined. HBV serological markers and HBV DNA were tested in 170 samples. Whole genome (n = 53) or specific region sequences: pre-S/S and basic core promoter/precore (BCP/PC) region (91 and 154 samples, respectively) were phylogenetically analyzed. The median age of infected donors was 21 years. Anti-HBs, anti-HBe and hepatitis B e antigen were detected in 5%, 92.4% and 10.5% of tested donors, respectively. The HBV DNA load ranged between unquantifiable and 3.1 x 10(10) IU/mL (median: 4.10 x 10(3) IU/mL). Genotypes A2 (81.2%) and D (18.8%) co-circulated. Phylogenetic analyses revealed differences between the genotypes. Viral load and level of HBsAg tended to be lower in genotype D. The median HBsAg/HBV DNA ratio expressed in IU/mL was one for both genotypes, but very low or very high ratios appeared more frequent in genotype D infections. Higher amino acid variability in the surface proteins (median: 4%vs 1.5%; P = 0.01) and in the major hydrophilic region was observed in genotype D (P = 0.01). BCP/PC region analysis revealed the double mutation 1762T/1764A in 49/125 (39.2%) genotype A2 and 6/29 (20.7%) genotype D strains (P = 0.08). Mutations in PC and BCP regions correlated neither with HBsAg nor HBV DNA levels. HBV genotype A2 is dominant in HBsAg positive donors in Poland. Minority genotype D strains are significantly more substituted than genotype A2 strains potentially affecting the course of infection.
乙型肝炎病毒（HBV）基因型具有明显的地理分布差异，并影响临床结局的严重程度和抗病毒治疗的反应。本研究检测了来自波兰的 HBsAg 阳性初次献血者中 HBV 表面抗原（HBsAg）的 HBV 多态性。在 170 个样本中检测了 HBV 血清学标志物和 HBV DNA。对 53 个全基因组（n = 53）或特定区域序列（91 个和 154 个样本分别为前 S/S 和基本核心启动子/前核心（BCP/PC）区）进行了系统发育分析。感染供者的中位年龄为 21 岁。在检测的供者中，分别有 5%、92.4%和 10.5%检测到抗-HBs、抗-HBe 和乙型肝炎 e 抗原。HBV DNA 载量范围为不可测至 3.1×10(10)IU/ml（中位数：4.10×10(3)IU/ml）。A2（81.2%）和 D（18.8%）基因型共同流行。系统发育分析显示基因型之间存在差异。基因型 D 的病毒载量和 HBsAg 水平较低。两种基因型的 HBsAg/HBV DNA 比值中位数均为 1IU/ml，但基因型 D 感染中出现低或高比值的频率更高。在表面蛋白（中位数：4%比 1.5%；P = 0.01）和主要亲水区观察到基因型 D 的氨基酸变异更高（P = 0.01）。BCP/PC 区分析显示，49/125（39.2%）基因型 A2 和 6/29（20.7%）基因型 D 株中存在双重突变 1762T/1764A（P = 0.08）。PC 和 BCP 区的突变与 HBsAg 或 HBV DNA 水平均无关。HBV 基因型 A2 在波兰 HBsAg 阳性供者中占优势。少数基因型 D 株的突变明显多于基因型 A2 株，这可能影响感染的过程。
