Wei J, Setlow P, Hoover D G
Department of Animal & Food Sciences, University of Delaware, Newark, DE 19716-2150, USA.
Lett Appl Microbiol. 2009 Nov;49(5):646-51. doi: 10.1111/j.1472-765X.2009.02721.x. Epub 2009 Aug 22.
To determine the germination and inactivation of Bacillus cereus spores lacking various germination proteins using moderately high pressure (MHP) and heat.
The inactivation and germination of wild-type B. cereus spores in buffer by MHP (150 MPa) at various temperatures, as well as the MHP inactivation and germination of B. cereus spores lacking individual germinant receptors and monovalent cation antiporters, was determined.
Loss of individual germinant receptors had no large effects on spore inactivation or germination, although germination of receptor-deficient spores was generally slightly decreased. Loss of the GerN in particular the GerN and GerT antiporters also decreased spore germination by MHP, especially at 40 and 50 degrees C.
Both inactivation and germination of B. cereus spores by MHP increased with rise of temperature; however, mutant strains lacking individual germinant receptor had similar levels of germination as compared to wild-type spores. To evaluate the role of germinant receptors in MHP, a strain lacking a large number of germinant receptors is needed.
The results of this work may lead to a better understanding of how MHP causes germination of spores of B. cereus.
使用适度高压（MHP）和加热来确定缺乏各种萌发蛋白的蜡样芽孢杆菌孢子的萌发和失活情况。
测定野生型蜡样芽孢杆菌孢子在缓冲液中于不同温度下经MHP（150兆帕）处理后的失活和萌发情况，以及缺乏单个萌发受体和单价阳离子反向转运蛋白的蜡样芽孢杆菌孢子的MHP失活和萌发情况。
单个萌发受体的缺失对孢子失活或萌发没有太大影响，尽管缺乏受体的孢子的萌发通常略有下降。特别是GerN的缺失，尤其是GerN和GerT反向转运蛋白的缺失，也会降低MHP处理下孢子的萌发，特别是在40和50摄氏度时。
蜡样芽孢杆菌孢子经MHP处理后的失活和萌发均随温度升高而增加；然而，与野生型孢子相比，缺乏单个萌发受体的突变菌株具有相似的萌发水平。为了评估萌发受体在MHP中的作用，需要一种缺乏大量萌发受体的菌株。
这项工作的结果可能有助于更好地理解MHP如何导致蜡样芽孢杆菌孢子的萌发。
