Lü Xin-quan, Suo Zhenhe, Ma Chang-lu, Xu Ke-jia, Liu Yi-shan, Li Hui-xiang
Department of Pathology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
Zhonghua Bing Li Xue Za Zhi. 2009 Jul;38(7):441-4.
To study the distribution and quantity of CD44+/CD24- cells in breast cancer tissue and the cell lines, and as well as its correlation with the expression of various breast cancer markers and molecular subtyping of breast carcinoma.
The expression of CD44/CD24, estrogen receptor, progesterone receptor, HER2, human estrogen-induced protein PS2, bcl-2 and nm23 in 60 cases of invasive ductal carcinoma of breast were studied by either single or double immunohistochemical staining. The co-expression of CD44 and CD24 in 3 breast cancer cell lines (MCF-7, MDA-MB-468, and MDA-MB-231) was also examined.
The quantity and distribution of CD44+/CD24- cells varied greatly and no specific patterns were identified. The percentage of CD44+/CD24- in breast cancer was 65%. The amount of CD44+/CD24- cells did not correlate with the age of patients, lymph node metastasis, tumor size, molecular subtypes and expression of various breast cancer markers in breast carcinoma. The proportion of CD44+/CD24- cells in MCF-7, MDA-MB-468, and MDA-MB-231 cell lines was <1%, 5% and >80%, respectively.
CD44+/CD24- cells are demonstrated in certain breast cancer tissues and cell lines. However, there is no relationship obtained between the quantity or the distribution of these cells and the molecular subtyping or the clinicopathologic parameters in breast cancer.
研究CD44+/CD24-细胞在乳腺癌组织及细胞系中的分布和数量，以及其与各种乳腺癌标志物表达和乳腺癌分子亚型的相关性。
采用单免疫组化或双免疫组化染色法，研究60例乳腺浸润性导管癌中CD44/CD24、雌激素受体、孕激素受体、HER2、人雌激素诱导蛋白PS2、bcl-2和nm23的表达。同时检测3种乳腺癌细胞系（MCF-7、MDA-MB-468和MDA-MB-231）中CD44和CD24的共表达情况。
CD44+/CD24-细胞的数量和分布差异很大，未发现特定模式。乳腺癌中CD44+/CD24-的比例为65%。CD44+/CD24-细胞的数量与患者年龄、淋巴结转移、肿瘤大小、分子亚型及乳腺癌各种标志物的表达均无相关性。在MCF-7、MDA-MB-468和MDA-MB-231细胞系中，CD44+/CD24-细胞的比例分别<1%、5%和>80%。
在某些乳腺癌组织和细胞系中证实存在CD44+/CD24-细胞。然而，这些细胞的数量或分布与乳腺癌的分子亚型或临床病理参数之间未发现相关性。
