[少突胶质细胞瘤中1p/19q染色体杂合性缺失与p53蛋白表达]

[Loss of heterozygosity of chromosome 1p/19q and p53 protein expression in oligodendroglioma].

作者信息

Xiong Ji, Liu Ying, Li Chao, Zhu Jing-jing, Ye Zhu-rong, Mao Ying, Wang Yin

机构信息

Department of Pathology, Shanghai Medical College of Fudan University, and Department of Neuropathology, Huashan Hospital, Shanghai 200032, China.

出版信息

Zhonghua Bing Li Xue Za Zhi. 2009 Jul;38(7):445-50.

PMID:19781190

Abstract

OBJECTIVE

To study the status of loss of heterozygosity (LOH) of chromosome 1p/19q and p53 protein expression in oligodendroglioma, as compared to astrocytoma.

METHODS

One hundred and ninety-one cases of glioma of different histologic types and grades, including 116 cases of low-grade of oligodendroglioma (86 paraffin-embedded and 30 fresh tissues), 45 cases of anaplastic oligodendroglioma (all paraffin-embedded tissues) and 30 cases of astrocytoma of various grades (all paraffin-embedded tissues), were enrolled into the study. The LOH of chromosome 1p/19q was investigated by polymerase chain reaction (PCR)-based microsatellite analysis. The p53 protein expression was demonstrated by immunohistochemical staining.

RESULTS

The rates of 1p loss, 19q loss and 1p/19q loss were 69.8%, 64%, and 57.0% respectively in the 86 paraffin-embedded low-grade oligodendroglioma samples, as compared to 71.1%, 60.0% and 55.6% respectively in the 45 paraffin-embedded anaplastic oligodendroglioma samples. There was no difference of LOH of 1p/19q between low-grade oligodendroglioma and anaplastic oligodendroglioma (P>0.05). In the 30 cases of low-grade oligodendroglioma with fresh tissues available, the rates of 1p loss, 19q loss and 1p/19q loss were 70.0%, 63.3% and 60.0% respectively. The LOH of 1p/19q between paraffin-embedded and fresh samples was not statistically significant (P>0.05). In the 30 cases of astrocytoma, the rates of 1p loss, 19q loss and 1p/19q loss were 23.3%, 33.3% and 20.0% respectively, which were significantly less than those in oligodendroglioma (P<0.05). The expression of p53 protein was significantly lower in low-grade oligodendroglioma (8.1%) than in anaplastic oligodendroglioma (31.1%, P=0.007). The expression of p53 protein in oligodendroglioma was also lower than in astrocytoma (P=0.001). Furthermore, p53 protein expression negatively correlated with 1p/19q loss in anaplastic oligodendroglioma (P<0.05).

CONCLUSIONS

Both paraffin-embedded and fresh tissues are suitable for analysis of LOH of chromosome 1p/19q. Oligodendroglioma demonstrates a higher frequency of LOH of chromosome 1p/19q and lower expression of p53 protein than astrocytoma. The LOH of chromosome 1p/19q negatively correlates with the expression of p53 protein. These parameters have both diagnostic and prognostic values.

摘要

目的

研究少突胶质细胞瘤中1p/19q杂合性缺失（LOH）及p53蛋白表达情况，并与星形细胞瘤作比较。

方法

纳入191例不同组织学类型和级别的胶质瘤病例，包括116例低级别少突胶质细胞瘤（86例石蜡包埋组织和30例新鲜组织）、45例间变性少突胶质细胞瘤（均为石蜡包埋组织）和30例不同级别的星形细胞瘤（均为石蜡包埋组织）。采用基于聚合酶链反应（PCR）的微卫星分析检测1p/19q染色体的LOH。通过免疫组织化学染色检测p53蛋白表达。

结果

86例石蜡包埋的低级别少突胶质细胞瘤样本中，1p缺失率、19q缺失率和1p/19q缺失率分别为69.8%、64%和57.0%；45例石蜡包埋的间变性少突胶质细胞瘤样本中，上述缺失率分别为71.1%、60.0%和55.6%。低级别少突胶质细胞瘤和间变性少突胶质细胞瘤之间1p/19q的LOH无差异（P>0.05）。在30例有新鲜组织的低级别少突胶质细胞瘤中，1p缺失率、19q缺失率和1p/19q缺失率分别为70.0%、63.3%和60.0%。石蜡包埋样本和新鲜样本之间1p/19q的LOH无统计学差异（P>0.05）。在30例星形细胞瘤中，1p缺失率、19q缺失率和1p/19q缺失率分别为23.3%、33.3%和20.0%，显著低于少突胶质细胞瘤（P<0.05）。低级别少突胶质细胞瘤中p53蛋白表达（8.1%）显著低于间变性少突胶质细胞瘤（31.1%，P=0.007）。少突胶质细胞瘤中p53蛋白表达也低于星形细胞瘤（P=0.001）。此外，间变性少突胶质细胞瘤中p53蛋白表达与1p/19q缺失呈负相关（P<0.05）。