硫化氢对离体大鼠心室肌细胞钾离子ATP通道电流的影响

[Effects of hydrogen sulfide on the K ATP current in isolated rat ventricular myocytes].

作者信息

Li Yan-bing, Zhong Guang-zhen, Yang Xin-chun, Liu Xiu-lan

机构信息

Department of Cardiology, Chaoyang Hospital, Capital Medical University, Beijing, China.

出版信息

Zhonghua Xin Xue Guan Bing Za Zhi. 2009 May;37(5):445-8.

PMID:19781223

Abstract

OBJECTIVE

To investigate the effects of endogenous and exogenous hydrogen sulfide (H(2)S) on the K(ATP) current in isolated rat ventricular myocytes.

METHODS

Ventricular myocytes were isolated from rat heart by modified Langendorff perfusion with collagenase. K(ATP) current of single rat ventricular myocytes was recorded by whole-cell patch-clamp technique.

RESULTS

The density of K(ATP) current was significantly reduced by 200 micromol/L DL-propargylglycine (PPG, an irreversible inhibitor of the H(2)S) [(5.3258 +/- 0.7556) pA/pF vs. (3.7856 +/- 0.4312) pA/pF, P < 0.01] in a time-dependent way. The density of K(ATP) current could be significantly increased by NaHS (a H(2)S donor, 9.375, 18.75, 37.5, 75, 150 micromol/L) in a concentration-dependent manner [(6.6310 +/- 0.6092) pA/pF vs. (9.0949 +/- 1.0259) pA/pF at 150 micromol/L, P < 0.01].

CONCLUSION

Both endogenous and exogenous H(2)S could open K(ATP) channels and enhance the K(ATP) current in rat ventricular myocytes.

摘要

目的

研究内源性和外源性硫化氢（H₂S）对离体大鼠心室肌细胞ATP敏感性钾电流（K(ATP)电流）的影响。

方法

采用改良Langendorff灌流法并用胶原酶从大鼠心脏分离心室肌细胞。用全细胞膜片钳技术记录单个大鼠心室肌细胞的K(ATP)电流。

结果

200 μmol/L DL-炔丙基甘氨酸（PPG，一种H₂S的不可逆抑制剂）可使K(ATP)电流密度以时间依赖性方式显著降低[（5.3258±0.7556）pA/pF对（3.7856±0.4312）pA/pF，P<0.01]。NaHS（一种H₂S供体，浓度为9.375、18.75、37.5、75、150 μmol/L）可使K(ATP)电流密度以浓度依赖性方式显著增加[（6.6310±0.6092）pA/pF对150 μmol/L时的（9.0949±1.0259）pA/pF，P<0.01]。