[Smads在瘢痕疙瘩形成中的表达]
[Expression of Smads in keloid scarring].
作者信息
Tang Bing, Zhu Bin, Bi Liang-Kuan, Xue Chun-Li, Cai Hao, Zhu Jia-Yuan
机构信息
Department of Burns, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
出版信息
Zhonghua Wai Ke Za Zhi. 2009 Jun 15;47(12):941-3.
OBJECTIVE
To investigate the differential expression of different types of Smads in keloids, normal scars and normal skins and its possible clinicopathological significance.
METHODS
RT-PCR and Western blot methods were used to examine the expression of Smads mRNA and proteins level in 10 cases of keloid, in 10 cases of normal scar and in 10 cases of normal skin tissues and fibroblasts. Fibroblasts of keloid, normal scar and normal skin were cultured in vitro. The expression difference were compared and analyzed by t-test, there was statistical difference when P < 0.05.
RESULTS
The mRNA and protein expression of inhibitory Smad7 were significantly down regulated in keloid compared with normal scar (P < 0.05) and normal skin (P < 0.05). However, no significant difference of the mRNA and protein expression of Smad2, 3 and the protein expression of phosphorylation of Smad2, 3 in keloid, normal scar, normal skin tissues and fibroblasts.
CONCLUSIONS
The decreased expression of Smad7 in keloid might play a significant role in the increased TGF-beta1/Smads signal transduction, which can not be terminated by autologous negative feedback cycle.
目的
研究不同类型的Smads在瘢痕疙瘩、正常瘢痕及正常皮肤中的差异表达及其可能的临床病理意义。
方法
采用逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法（Western blot）检测10例瘢痕疙瘩、10例正常瘢痕及10例正常皮肤组织和成纤维细胞中Smads mRNA及蛋白水平。体外培养瘢痕疙瘩、正常瘢痕及正常皮肤的成纤维细胞。采用t检验比较分析表达差异，P<0.05为差异有统计学意义。
结果
与正常瘢痕（P<0.05）和正常皮肤（P<0.05）相比，瘢痕疙瘩中抑制性Smad7的mRNA和蛋白表达显著下调。然而，瘢痕疙瘩、正常瘢痕、正常皮肤组织和成纤维细胞中Smad2、3的mRNA和蛋白表达以及Smad2、3磷酸化蛋白表达无显著差异。
结论
瘢痕疙瘩中Smad7表达降低可能在转化生长因子-β1（TGF-β1）/Smads信号转导增强中起重要作用，且不能通过自身负反馈循环终止。
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