Instituto Nacional del Carbón (CSIC), Apartado 73, 33080-Oviedo, Spain.
J Colloid Interface Sci. 2009 Dec 15;340(2):230-6. doi: 10.1016/j.jcis.2009.09.003. Epub 2009 Sep 6.
A synthetic method for the fabrication of silica-based mesoporous magnetic (Fe or iron oxide spinel) nanocomposites with enhanced adsorption and magnetic capabilities is presented. The successful in situ synthesis of magnetic nanoparticles is a consequence of the incorporation of a small amount of carbon into the pores of the silica, this step being essential for the generation of relatively large iron oxide magnetic nanocrystals ( approximately 10+/-3nm) and for the formation of iron nanoparticles. These composites combine good magnetic properties (superparamagnetic behaviour in the case of SiO(2)-C-Fe(3)O(4)/gamma-Fe(2)O(3) samples) with a large and accessible porosity made up of wide mesopores (>9nm). In the present work, we have demonstrated the usefulness of this kind of composite for the adsorption of a globular protein (hemoglobin). The results obtained show that a significant amount of hemoglobin can be immobilized within the pores of these materials (up to 180mgg(-1) for some of the samples). Moreover, we have proved that the composite loaded with hemoglobin can be easily manipulated by means of an external magnetic field.
一种用于制备具有增强吸附和磁性能的基于二氧化硅的介孔磁性（铁或铁氧化物尖晶石）纳米复合材料的合成方法。磁性纳米粒子的成功原位合成是由于在二氧化硅的孔中掺入少量碳，这一步骤对于生成相对较大的氧化铁磁性纳米晶（约 10+/-3nm）和形成铁纳米粒子至关重要。这些复合材料结合了良好的磁性能（SiO(2)-C-Fe(3)O(4)/γ-Fe(2)O(3 样品表现出超顺磁性行为）和由大且可及的孔组成的大孔，这些孔由宽介孔（>9nm）组成。在本工作中，我们已经证明了这种复合材料在吸附球蛋白（血红蛋白）方面的有用性。所得到的结果表明，可以将相当大量的血红蛋白固定在这些材料的孔内（对于一些样品，可达 180mgg(-1)）。此外，我们已经证明，负载有血红蛋白的复合材料可以通过外部磁场轻松操作。
