International Center for Young Scientists (ICYS), National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba, Ibaraki 305-0047, Japan.
Small. 2010 Feb 5;6(3):471-8. doi: 10.1002/smll.200901403.
Rattle-type Fe(3)O(4)@SiO(2) hollow mesoporous spheres with different particle sizes, different mesoporous shell thicknesses, and different levels of Fe(3)O(4) content are prepared by using carbon spheres as templates. The effects of particle size and concentration of Fe(3)O(4)@SiO(2) hollow mesoporous spheres on cell uptake and their in vitro cytotoxicity to HeLa cells are evaluated. The spheres exhibit relatively fast cell uptake. Concentrations of up to 150 microg mL(-1) show no cytotoxicity, whereas a concentration of 200 microg mL(-1) shows a small amount of cytotoxicity after 48 h of incubation. Doxorubicin hydrochloride (DOX), an anticancer drug, is loaded into the Fe(3)O(4)@SiO(2) hollow mesoporous spheres, and the DOX-loaded spheres exhibit a somewhat higher cytotoxicity than free DOX. These results indicate the potential of Fe(3)O(4)@SiO(2) hollow mesoporous spheres for drug loading and delivery into cancer cells to induce cell death.
不同粒径、不同介孔壳厚度和不同 Fe(3)O(4)含量的类铃型 Fe(3)O(4)@SiO(2)中空介孔球，是以碳球为模板制备的。评价了 Fe(3)O(4)@SiO(2)中空介孔球的粒径和浓度对细胞摄取的影响及其对 HeLa 细胞的体外细胞毒性。这些球表现出相对较快的细胞摄取。高达 150 μg mL(-1)的浓度没有细胞毒性，而在孵育 48 小时后，浓度为 200 μg mL(-1)时显示出少量细胞毒性。盐酸阿霉素（DOX），一种抗癌药物，被载入 Fe(3)O(4)@SiO(2)中空介孔球中，载 DOX 的球显示出比游离 DOX 稍高的细胞毒性。这些结果表明 Fe(3)O(4)@SiO(2)中空介孔球具有将药物载入并递送至癌细胞以诱导细胞死亡的潜力。
