Singapore Eye Research Institute, Singapore, Singapore.
Biomaterials. 2010 Jan;31(2):216-25. doi: 10.1016/j.biomaterials.2009.09.034. Epub 2009 Sep 24.
In the past 20 years, human amniotic membrane (AM) has become widely used as an ophthalmic surgical patch as well as a substrate for stem cell tissue equivalents for ocular surface reconstruction. AM reduces ocular surface scarring and inflammation, and enhances epithelialization. In addition, it shows limited immunogenicity and some anti-microbial properties. Before being applied clinically, the donor of AM is required to undergo a thorough health screening and the membrane has to undergo an accepted processing routine, which includes preservation, sterilization and de-epithelialization. There have been various articles describing methods in preserving, sterilizing and de-epithelializing AM. Each preparation technique has been reported to have differential effects on the physical and biological properties of the AM. Therefore, it is difficult to establish a standardized procedure. In this review, we discuss the present techniques and several novel, new approaches in the preparation of AM for use in ocular surface reconstruction, and their impact on AM structure and biological activity.
在过去的 20 年中，人羊膜（AM）已广泛用作眼科手术贴片以及用于眼表面重建的干细胞组织等效物的基底。AM 可减少眼表面瘢痕和炎症，并促进上皮化。此外，它显示出有限的免疫原性和一些抗微生物特性。在临床应用之前，需要对 AM 的供体进行彻底的健康筛查，并且必须对膜进行公认的处理常规，包括保存，消毒和去上皮化。已经有各种文章描述了保存，消毒和去上皮化 AM 的方法。据报道，每种制备技术对 AM 的物理和生物学特性均有不同的影响。因此，很难建立标准化程序。在这篇综述中，我们讨论了目前用于眼表面重建的 AM 制备的技术和几种新颖的新方法，以及它们对 AM 结构和生物活性的影响。
