Uyeno E T, Davies M F, Pryor G T, Loew G H
Life Sciences Division, SRI International, Menlo Park, CA 94025.
Life Sci. 1990;47(15):1375-82. doi: 10.1016/0024-3205(90)90202-3.
The punished drinking test has been used successfully for identifying and studying anxiolytic agents. By reducing the level of punishment (i.e., decreasing the intensity of shock), it has also been used as a method for measuring anxiogenic activity. Because anxiogenic behavior is a novel and important concept that is not yet fully established, we have reinvestigated the effects of two putative inverse benzodiazepine agonists and pentylenetetrazol in this conflict test. In a series of experiments, using both our version of the procedure and a replication of a previously published method, we were unable to demonstrate a selective reduction in punished responding over unpunished responding caused by CGS 8216 (3 to 40 mg/kg), FG 7142 (2 to 6 mg/kg), and pentylenetetrazol (10 to 20 mg/kg) as reported previously. A careful comparison of the details of our method and the published procedure failed to reveal the source of this discrepancy. If anxiogenic behavior is to be defined as a selective effect of a drug on punished response, the value of this test will depend on identification of its critical variables.
惩罚性饮酒试验已成功用于识别和研究抗焦虑药物。通过降低惩罚水平（即降低电击强度），它也被用作测量致焦虑活性的一种方法。由于致焦虑行为是一个尚未完全确立的新颖且重要的概念，我们在这个冲突试验中重新研究了两种假定的反向苯二氮䓬激动剂和戊四氮的作用。在一系列实验中，使用我们的实验程序版本以及重复先前发表的方法，我们无法证明如先前报道的那样，CGS 8216（3至40毫克/千克）、FG 7142（2至6毫克/千克）和戊四氮（10至20毫克/千克）会导致惩罚反应相对于非惩罚反应有选择性降低。对我们的方法细节与已发表程序进行仔细比较，未能揭示这种差异的来源。如果致焦虑行为要被定义为药物对惩罚反应的选择性作用，那么这个试验的价值将取决于其关键变量的识别。
