Chemistry Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.
Talanta. 2009 Nov 15;80(1):151-7. doi: 10.1016/j.talanta.2009.06.042. Epub 2009 Jun 25.
Studies were carried out, for the first time, to investigate the charge-transfer reactions of ganciclovir as n-electron donor with the sigma-acceptor iodine and various pi-acceptors: 7,7,8,8-tetracyanoquinodimethane; tetracyanoethylene; 2,3-dichloro-5,6-dicyano-1,4-benzoquinone; p-chloranilic acid; 2,3,5,6-tetrabromo-1,4-benzoquinone; 2,3,5,6-tetrachloro-1,4-benzoquinone and 2,4,7-trinitro-9-fluorenone. The formation of the colored charge-transfer complexes was utilized in the development of simple, rapid and accurate spectrophotometric methods for the analysis of ganciclovir in pure form as well as in its pharmaceutical formulation (capsules). Different variables affecting the reactions were studied and optimized. Under the optimum reaction conditions, linear relationships with good correlation coefficients (0.9993-0.9998) were found between the absorbance and the concentration of ganciclovir in the range of 2.0-240 microg mL(-1). For more accurate analysis, Ringbom optimum concentration range was found to be between 5.0 and 225 microg mL(-1). The limits of detection ranged from 0.36 to 2.45 microg mL(-1) and the limits of quantification ranged from 1.20 to 8.17 microg mL(-1). A Job's plot of the absorbance versus the molar ratio of ganciclovir to each of acceptors under consideration indicated (1:1) ratio. The proposed methods were applied successfully for simultaneous determination of ganciclovir in capsules with good accuracy and precision and without interferences from common additives. The recovery percentages ranged from 99.45+/-0.73% to 100.35+/-1.40%. The results were compared favourably with the reported method.
首次进行了研究，以调查更昔洛韦作为 n-电子供体与 sigma-受体碘和各种 pi-受体：7,7,8,8-四氰基醌二甲烷；四氰乙烯；2,3-二氯-5,6-二氰基-1,4-苯醌；对氯邻苯二甲酸；2,3,5,6-四溴-1,4-苯醌；2,3,5,6-四氯-1,4-苯醌和 2,4,7-三硝基-9-芴酮的电荷转移反应。利用形成有色电荷转移配合物，开发了简单、快速和准确的分光光度法，用于分析更昔洛韦的纯品以及其药物制剂（胶囊）。研究并优化了影响反应的不同变量。在最佳反应条件下，在 2.0-240μgmL(-1)的范围内，吸光度与更昔洛韦浓度之间存在良好的线性关系，相关系数为 0.9993-0.9998。为了更准确的分析，发现环棒最佳浓度范围在 5.0-225μgmL(-1)之间。检测限范围为 0.36-2.45μgmL(-1)，定量限范围为 1.20-8.17μgmL(-1)。更昔洛韦与各受体摩尔比的吸收度工作图表明（1:1）比值。该方法成功应用于胶囊中更昔洛韦的同时测定，具有良好的准确性和精密度，且无常见添加剂的干扰。回收率范围为 99.45+/-0.73%至 100.35+/-1.40%。结果与报道的方法相比具有优越性。
