Key Laboratory of the Ministry of Education for Experimental Teratology, Department of Histology and Embryology, Shandong University School of Medicine, Jinan, 250012 Shandong, China.
Neuroscience. 2009 Dec 29;164(4):1521-30. doi: 10.1016/j.neuroscience.2009.09.045. Epub 2009 Sep 24.
Granulocyte-colony stimulating factor (G-CSF) is a growth factor that regulates proliferation, differentiation and survival of hematopoietic progenitor cells. There is growing evidence to suggest that G-CSF exerts a powerful neuroprotective effect in different neurological disorders. However, it has remained to be elucidated if G-CSF has a direct effect on neural stem cells (NSCs). Here, we show that G-CSF could stimulate the proliferation of NSCs and promote their differentiation in vitro. Additionally, we have shown that G-CSF-induced proliferation of NSCs is associated with phosphorylation of STAT3, and the differentiation is linked to altered expression of differentiation-related genes. Remarkably, G-CSF could not initiate the differentiation of NSCs. The added roles of G-CSF in regulating proliferation and differentiation of NSCs as shown in this study would serve as a useful reference in designing new stem cell therapy strategies for promoting brain recovery and repair.
粒细胞集落刺激因子（G-CSF）是一种调节造血祖细胞增殖、分化和存活的生长因子。越来越多的证据表明，G-CSF 在不同的神经紊乱中发挥强大的神经保护作用。然而，G-CSF 是否对神经干细胞（NSCs）有直接作用仍有待阐明。在这里，我们发现 G-CSF 可以刺激 NSCs 的增殖，并促进其体外分化。此外，我们还表明，G-CSF 诱导的 NSCs 增殖与 STAT3 的磷酸化有关，而分化与分化相关基因的表达改变有关。值得注意的是，G-CSF 不能启动 NSCs 的分化。本研究表明，G-CSF 在调节 NSCs 增殖和分化中的附加作用将为设计新的干细胞治疗策略以促进大脑恢复和修复提供有用的参考。
