He Chunsheng, Zhu Weiping, Xu Yufang, Chen Tao, Qian Xuhong
Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
Anal Chim Acta. 2009 Oct 5;651(2):227-33. doi: 10.1016/j.aca.2009.09.006. Epub 2009 Sep 6.
In this work, a reusable bifunctional fluorescent sensor for simultaneous detection and separation of trace Hg2+ in water and serum, which contains a naphthalimide derivative of 2,6-bis(aminomethyl)pyridine covalently grafted to the surface of silica particles, was developed. Meanwhile, the fluorescence characteristics and the adsorbent properties of the sensor were investigated in detail. This sensor showed a very good linearity (correlation coefficient of R2=0.9991) in the range 0.1-1 microM of Hg2+ with detection limits lower than 6.8 x 10(-9) M. It can also be used as an adsorbent for the removal of mercuric ions from the contaminated aqueous solution. The regeneration of this sensor is very simple, only by modulating the pH value of the aqueous solution. It can be reused at least four cycles. In addition, the present approach has the advantages of rapidity, simplicity, and low cost. We believe that this approach may serve as a foundation for the preparation of practical fluorescent sensor for the rapid detection of Hg2+ in aqueous biological and environmental samples.
在这项工作中，开发了一种可重复使用的双功能荧光传感器，用于同时检测和分离水和血清中的痕量Hg2+，该传感器包含共价接枝到二氧化硅颗粒表面的2,6-双（氨甲基）吡啶的萘酰亚胺衍生物。同时，详细研究了该传感器的荧光特性和吸附性能。该传感器在0.1-1 microM的Hg2+范围内表现出非常好的线性（相关系数R2 = 0.9991），检测限低于6.8×10（-9）M。它还可以用作吸附剂，用于从受污染的水溶液中去除汞离子。该传感器的再生非常简单，只需调节水溶液的pH值即可。它至少可以重复使用四个循环。此外，本方法具有快速、简单和低成本的优点。我们相信，这种方法可为制备用于快速检测生物和环境水样中Hg2+的实用荧光传感器奠定基础。
