Laboratório de Processos Biológicos, Departamento de Hidráulica e Saneamento, Escola de Engenharia de São Carlos, Universidade de São Paulo, Engenharia Ambiental-Bloco 4-F, Av. João Dagnone, 1100-Santa Angelina, 13.563-120, São Carlos, SP, Brazil.
Bioresour Technol. 2010 Feb;101(4):1324-30. doi: 10.1016/j.biortech.2009.09.026. Epub 2009 Sep 26.
The objective of this work was to evaluate the influence of different carbon sources and the carbon/nitrogen ratio (C/N) on the production and main composition of insoluble extracellular polymers (EPS) produced in an anaerobic sequencing batch biofilm reactor (ASBBR) with immobilized biomass in polyurethane foam. The yield of EPS was 23.6 mg/g carbon, 13.3 mg/g carbon, 9.0 mg/g carbon and 1.4 mg/g carbon when the reactor was fed with glucose, soybean oil, fat acids, and meat extract, respectively. The yield of EPS decreased from 23.6 to 2.6 mg/g carbon as the C/N ratio was decreased from 13.6 to 3.4 gC/gN, using glucose as carbon source. EPS production was not observed under strict anaerobic conditions. The results suggest that the carbon source, microaerophilic conditions and high C/N ratio favor EPS production in the ASBBR used for wastewater treatment. Cellulose was the main exopolysaccharide observed in all experimental conditions.
本工作旨在评估不同碳源和碳氮比（C/N）对固定化生物量在聚氨酯泡沫中的厌氧序批式生物膜反应器（ASBBR）中产生的不可溶性胞外聚合物（EPS）的产量和主要成分的影响。当反应器分别以葡萄糖、大豆油、脂肪酸和肉膏作为碳源时，EPS 的产率分别为 23.6、13.3、9.0 和 1.4mg/g 碳。当 C/N 比从 13.6 降低至 3.4gC/gN 时，以葡萄糖作为碳源，EPS 的产率从 23.6 降低至 2.6mg/g 碳。在严格的厌氧条件下未观察到 EPS 的产生。结果表明，对于用于废水处理的 ASBBR，碳源、微需氧条件和高 C/N 比有利于 EPS 的产生。在所有实验条件下均观察到纤维素是主要的胞外多糖。
